Although the first ancient DNA molecules were extracted more than three decades ago, the
first ancient nuclear genomes could only be characterized after high-throughput sequencing …

The approximate 30 extant invertebrate phyla have diversified along separate evolutionary
trajectories for hundreds of millions of years. Although recent work understandably has …

Whole-genome sequencing has become an indispensible tool of modern biology. However,
the cost of sample preparation relative to the cost of sequencing remains high, especially for …

It has been shown that highly fragmented DNA is most efficiently converted into DNA
libraries for sequencing if both strands of the DNA fragments are processed independently …

Sequencing of 16S rRNA gene tags is a popular method for profiling and comparing
microbial communities. The protocols and methods used, however, vary considerably with …

Motivation: Chimeric DNA sequences often form during polymerase chain reaction
amplification, especially when sequencing single regions (eg 16S rRNA or fungal Internal …

The advent of next generation sequencing has coincided with a growth in interest in using
these approaches to better understand the role of the structure and function of the microbial …

Bacterial diversity among environmental samples is commonly assessed with PCR-
amplified 16S rRNA gene (16S) sequences. Perceived diversity, however, can be influenced …

Intestinal microbiota are critical for health with changes associated with diverse human
diseases. Research suggests that altered intestinal microbiota can profoundly affect brain …

Droplet-based scRNA-seq assays are known to produce a significant amount of background
RNA counts, the hallmark of which is non-zero transcript counts in presumably empty …