Onsite detection of plant viruses using isothermal amplification assays

AI Bhat, R Aman, M Mahfouz - Plant biotechnology journal, 2022 - Wiley Online Library
Plant diseases caused by viruses limit crop production and quality, resulting in significant
losses. However, options for managing viruses are limited; for example, as systemic obligate …

Molecular approaches for low-cost point-of-care pathogen detection in agriculture and forestry

P Baldi, N La Porta - Frontiers in Plant Science, 2020 - frontiersin.org
Early detection of plant diseases is a crucial factor to prevent or limit the spread of a rising
infection that could cause significant economic loss. Detection test on plant diseases in the …

Rapid and sensitive detection of potato virus X by one-step reverse transcription-recombinase polymerase amplification method in potato leaves and dormant tubers

R Kumar, P Kaundal, RK Tiwari, S Siddappa… - Molecular and cellular …, 2021 - Elsevier
Potato virus X (PVX), is a serious threat to global potato production. A simple and rapid
detection method is imperative for PVX diagnosis and early management. In this study, an …

Recombinase polymerase amplification applied to plant virus detection and potential implications

B Babu, FM Ochoa-Corona, ML Paret - Analytical Biochemistry, 2018 - Elsevier
Several isothermal techniques for the detection of plant pathogens have been developed
with the advent of molecular techniques. Among them, Recombinase Polymerase …

The complex world of emaraviruses—Challenges, insights, and prospects

M Rehanek, DG Karlin, M Bandte, R Al Kubrusli… - Forests, 2022 - mdpi.com
Emaravirus (Order Bunyavirales; Family Fimoviridae) is a genus comprising over 20
emerging plant viruses with a worldwide distribution and economic impact. Emaraviruses …

Current status of recombinase polymerase amplification technologies for the detection of pathogenic microorganisms

S Zhang, M Duan, S Li, J Hou, T Qin, Z Teng… - … and infectious disease, 2024 - Elsevier
Rapid detection of pathogenic microorganisms is key to the epidemiologic identification,
prevention and control of disease in the field of public health. PCR-based pathogen …

Development of a multiplex RT‐RPA assay for simultaneous detection of three viruses in cucurbits

AAK Jailani, ML Paret - Molecular Plant Pathology, 2023 - Wiley Online Library
Begomoviruses and criniviruses, vectored by whiteflies (Bemisia tabaci), are important
threats to crops worldwide. In recent years, the spread of cucurbit leaf crumple virus …

Recombinase polymerase amplification assay for field detection of tomato bacterial spot pathogens

A Strayer-Scherer, JB Jones, ML Paret - Phytopathology, 2019 - Am Phytopath Society
Bacterial spot of tomato is caused by Xanthomonas gardneri, X. euvesicatoria, X. perforans,
and X. vesicatoria. Current diagnostic methods for the pathogens are not in-field assays …

Visual DNA diagnosis of Tomato yellow leaf curl virus with integrated recombinase polymerase amplification and a gold-nanoparticle probe

TM Wang, JT Yang - Scientific reports, 2019 - nature.com
A visual DNA diagnosis with a rapid and simple procedure has been developed on
integrating recombinase polymerase amplification (RPA) and a gold nanoparticle (AuNP) …

Development of a reverse genetic system for studying rose rosette virus in whole plants

J Verchot, V Herath, CD Urrutia… - Molecular Plant …, 2020 - Am Phytopath Society
Rose rosette virus (RRV) is a negative-sense RNA virus with a seven-segmented genome
that is enclosed by a double membrane. We constructed an unconventional minireplicon …