Fluorescence nanoscopy uniquely combines minimally invasive optical access to the
internal nanoscale structure and dynamics of cells and tissues with molecular detection …

The emerging inverse electron demand Diels–Alder (IEDDA) reaction stands out from other
bioorthogonal reactions by virtue of its unmatchable kinetics, excellent orthogonality and …

BACKGROUND Biomolecular mechanisms are typically inferred from static structural
“snapshots” obtained by x-ray crystallography, nuclear magnetic resonance (NMR) …

Over the past 16 years, genetic code expansion and reprogramming in living organisms has
been transformed by advances that leverage the unique properties of pyrrolysyl-tRNA …

Fluorescence resonance energy transfer (FRET) is a powerful spectroscopic method for
measuring distances in the 2–8 nm range. Often, conformational changes and molecular …

Over the past years, fluorescent proteins (eg, green fluorescent proteins) have been widely
utilized to visualize recombinant protein expression and localization in live cells. Although …

Conspectus Expansion of the genetic code allows unnatural amino acids (Uaas) to be site-
specifically incorporated into proteins in live biological systems, thus enabling novel …

The superfamily of G protein-coupled receptors (GPCRs) mediates a wide range of
physiological responses and serves as an important category of drug targets. Earlier …

The pyrrolysyl-tRNA/pyrrolysyl-tRNA synthetase (PylT/RS) pair from the archaeon
Methanosarcina mazei (Mma) is widely used in protein engineering to site-specifically …

Unnatural amino acids (UAAs) have gained significant attention in protein engineering and
drug development owing to their ability to introduce new chemical functionalities to proteins …