Replication of the two template strands at eukaryotic cell DNA replication forks is a highly
coordinated process that ensures accurate and efficient genome duplication. Biochemical …

▪ Abstract One strand of cellular DNA is generated as RNA-initiated discontinuous segments
called Okazaki fragments that later are joined. The RNA terminated region is displaced into …

Flap endonucleases (FENs) isolated from archaea are shown to recognize and cleave a
structure formed when two overlapping oligonucleotides hybridize to a target DNA strand …

Unlike the most well‐characterized prokaryotic polymerase, E. Coli DNA pol I, none of the
eukaryotic polymerases have their own 5′ to 3′ exonuclease domain for nick translation …

Many recent reviews of eukaryotic DNA replication have emphasized our current
understanding of either the initiation and regulation of eukaryotic nuclear DNA replication (1 …

Completion of lagging strand DNA synthesis requires processing of up to 50 million Okazaki
fragments per cell cycle in mammalian cells. Even in yeast, the Okazaki fragment maturation …

Base damage or loss occurs at high frequency in the cells (almost 104 bases are damaged
and hydrolysed per cell per day). DNA repair is fundamental to maintain genomic integrity …

The 5′> 3′-exonuclease domain of Escherichia coli DNA polymerase I is required for the
completion of lagging strand DNA synthesis, and yet this domain is not present in any of the …

Structure-specific nucleases catalyze critical reactions in DNA replication, recombination,
and repair. Recently, a structure-specific endonuclease, FEN-1, has been purified and …

Abstract Flap endonuclease (FEN-1) removes 5′ overhanging flaps in DNA repair and
processes the 5′ ends of Okazaki fragments in lagging strand DNA synthesis. The crystal …