[HTML][HTML] High-content CRISPR screening
CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …
interrogation of gene function in a wide range of applications and species. In pooled …
[HTML][HTML] CRISPR in cancer biology and therapy
Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …
transforming biomedical research and providing entirely new approaches for dissecting all …
[HTML][HTML] TDP-43 loss and ALS-risk SNPs drive mis-splicing and depletion of UNC13A
Variants of UNC13A, a critical gene for synapse function, increase the risk of amyotrophic
lateral sclerosis and frontotemporal dementia,–, two related neurodegenerative diseases …
lateral sclerosis and frontotemporal dementia,–, two related neurodegenerative diseases …
[HTML][HTML] Enhanced prime editing systems by manipulating cellular determinants of editing outcomes
While prime editing enables precise sequence changes in DNA, cellular determinants of
prime editing remain poorly understood. Using pooled CRISPRi screens, we discovered that …
prime editing remain poorly understood. Using pooled CRISPRi screens, we discovered that …
[HTML][HTML] The CRISPR-Cas toolbox and gene editing technologies
G Liu, Q Lin, S Jin, C Gao - Molecular cell, 2022 - cell.com
The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …
technologies, which are widely used in the life sciences. To improve the performance of …
Meningioma DNA methylation groups identify biological drivers and therapeutic vulnerabilities
Meningiomas are the most common primary intracranial tumors. There are no effective
medical therapies for meningioma patients, and new treatments have been encumbered by …
medical therapies for meningioma patients, and new treatments have been encumbered by …
Epigenetic regulation of T cell exhaustion
Chronic antigen stimulation during viral infections and cancer can lead to T cell exhaustion,
which is characterized by reduced effector function and proliferation, and the expression of …
which is characterized by reduced effector function and proliferation, and the expression of …
[HTML][HTML] Genome-wide CRISPR screens of T cell exhaustion identify chromatin remodeling factors that limit T cell persistence
T cell exhaustion limits antitumor immunity, but the molecular determinants of this process
remain poorly understood. Using a chronic stimulation assay, we performed genome-wide …
remain poorly understood. Using a chronic stimulation assay, we performed genome-wide …
[HTML][HTML] Engineering the next-generation of CAR T-cells with CRISPR-Cas9 gene editing
A Dimitri, F Herbst, JA Fraietta - Molecular cancer, 2022 - Springer
Abstract Chimeric Antigen Receptor (CAR) T-cells represent a breakthrough in personalized
cancer therapy. In this strategy, synthetic receptors comprised of antigen recognition …
cancer therapy. In this strategy, synthetic receptors comprised of antigen recognition …
[HTML][HTML] Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing
A general approach for heritably altering gene expression has the potential to enable many
discovery and therapeutic efforts. Here, we present CRISPRoff—a programmable epigenetic …
discovery and therapeutic efforts. Here, we present CRISPRoff—a programmable epigenetic …