Throughout its 40-year history, the field of gene therapy has been marked by many
transitions. It has seen great strides in combating human disease, has given hope to patients …

Retroviruses are distinguished from other viruses by two characteristic steps in the viral
replication cycle. The first is reverse transcription, which results in the production of a double …

HIV-1 integration into the host genome favors actively transcribed genes. Prior work
indicated that the nuclear periphery provides the architectural basis for integration site …

A hallmark of retroviral replication is establishment of the proviral state, wherein a DNA copy
of the viral RNA genome is stably incorporated into a host cell chromosome. Integrase is the …

Pioneering gene therapy trials have shown that the genetic engineering of haematopoietic
stem and progenitor cells can be an alternative to allogeneic transplantation in the treatment …

Integration is vital to retroviral replication and influences the establishment of the latent HIV
reservoir. HIV-1 integration favors active genes, which is in part determined by the …

The integration of a DNA copy of the viral RNA genome into host chromatin is the defining
step of retroviral replication. This enzymatic process is catalyzed by the virus-encoded …

Increasing evidence suggests that chromatin modifications have important roles in
modulating constitutive or alternative splicing. Here we demonstrate that the PWWP domain …

We have analyzed host cell genes linked to HIV replication that were identified in nine
genome-wide studies, including three independent siRNA screens. Overlaps among the …

Chimeric antigen receptor–engineered T cells targeting CD19 (CART19) provide an
effective treatment for pediatric acute lymphoblastic leukemia but are less effective for …