Bioorthogonal chemistry represents a class of high-yielding chemical reactions that proceed
rapidly and selectively in biological environments without side reactions towards …

In the realm of biological research, the invention of super-resolution microscopy (SRM) has
enabled the visualization of ultrafine sub-cellular structures and their functions in live cells at …

Cells in complex organisms undergo frequent functional changes, but few methods allow
comprehensive longitudinal profiling of living cells. Here we introduce scission-accelerated …

The tetrazine/trans-cyclooctene ligation stands out from the bioorthogonal toolbox due to its
exceptional reaction kinetics, enabling multiple molecular technologies in vitro and in living …

Single‐molecule localization microscopy (SMLM) has found extensive applications in
various fields of biology and chemistry. As a vital component of SMLM, fluorophores play an …

Advancement of bioorthogonal chemistry in molecular optical imaging lies in expanding the
repertoire of fluorophores that can undergo fluorescence signal changes upon …

Decoding cellular processes requires visualization of the spatial distribution and dynamic
interactions of biomolecules. It is therefore not surprising that innovations in imaging …

Bioorthogonal fluorogenic dyes are indispensable tools in wash‐free bioimaging of specific
biological targets. However, the fluorogenicity of existing tetrazine‐based bioorthogonal …

Live-cell single-molecule localization microscopy has advanced with the development of self-
blinking rhodamines. A p K cycling of< 6 is recognized as the criterion for self-blinking, yet a …

Small-molecule fluorophores enable the observation of biomolecules in their native context
with fluorescence microscopy. Specific labeling via bio-orthogonal tetrazine chemistry …