Screening for novel enzymes for biocatalytic processes: accessing the metagenome as a resource of novel functional sequence space
P Lorenz, K Liebeton, F Niehaus, J Eck - Current opinion in biotechnology, 2002 - Elsevier
Historically, biotechnology has missed up to 99% of existing microbial resources by using
traditional screening techniques. Strategies of directly cloning 'environmental …
traditional screening techniques. Strategies of directly cloning 'environmental …
Strategies for accessing soil metagenome for desired applications
J Rajendhran, P Gunasekaran - Biotechnology advances, 2008 - Elsevier
Most of the microorganisms in nature are inaccessible as they are uncultivable in the
laboratory. Metagenomic approaches promise the accessibility of the genetic resources and …
laboratory. Metagenomic approaches promise the accessibility of the genetic resources and …
Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples
Advances in the sequencing of DNA extracted from media such as soil and water offer huge
opportunities for biodiversity monitoring and assessment, particularly where the collection or …
opportunities for biodiversity monitoring and assessment, particularly where the collection or …
Extraction of DNA from soil
P Robe, R Nalin, C Capellano, TM Vogel… - European Journal of Soil …, 2003 - Elsevier
There is an increased interest in the extraction of nucleic acids from various environmental
samples, since molecular techniques allow less biased access to a greater portion of …
samples, since molecular techniques allow less biased access to a greater portion of …
Monitoring and source tracking of tetracycline resistance genes in lagoons and groundwater adjacent to swine production facilities over a 3-year period
S Koike, IG Krapac, HD Oliver… - Applied and …, 2007 - Am Soc Microbiol
To monitor the dissemination of resistance genes into the environment, we determined the
occurrence of tetracycline resistance (Tcr) genes in groundwater underlying two swine …
occurrence of tetracycline resistance (Tcr) genes in groundwater underlying two swine …
Nucleic acid purification using microfabricated silicon structures
NC Cady, S Stelick, CA Batt - Biosensors and Bioelectronics, 2003 - Elsevier
A microfluidic device has been designed, fabricated and tested for its ability to purify
bacteriophage lambda DNA and bacterial chromosomal DNA, a necessary prerequisite for …
bacteriophage lambda DNA and bacterial chromosomal DNA, a necessary prerequisite for …
Evaluation of extraction and purification methods for obtaining PCR-amplifiable DNA from compost for microbial community analysis
Analysis of microbial community structure in complex environmental samples using nucleic
acid techniques requires efficient unbiased DNA extraction procedures; however, humic …
acid techniques requires efficient unbiased DNA extraction procedures; however, humic …
Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea
X Lai, L Cao, H Tan, S Fang, Y Huang… - The ISME …, 2007 - academic.oup.com
To elucidate fungal diversity in methane hydrate-bearing deep-sea marine sediments in the
South China Sea, internal transcribed spacer (ITS) regions of rRNA genes from five different …
South China Sea, internal transcribed spacer (ITS) regions of rRNA genes from five different …
[PDF][PDF] Assessing PCR inhibition from humic substances
CD Matheson, C Gurney, N Esau… - The Open Enzyme …, 2010 - researchgate.net
Inhibition remains the greatest methodological challenge in molecular analysis of buried
biological remains. Inhibitory compounds associated with soil environments comprise …
biological remains. Inhibitory compounds associated with soil environments comprise …
Tetracycline residues and tetracycline resistance genes in groundwater impacted by swine production facilities
RI Mackie, S Koike, I Krapac, J Chee-Sanford… - Animal …, 2006 - Taylor & Francis
Antibiotics are used at therapeutic levels to treat disease; at slightly lower levels as
prophylactics; and at low, subtherapeutic levels for growth promotion and improvement of …
prophylactics; and at low, subtherapeutic levels for growth promotion and improvement of …