Screening for novel enzymes for biocatalytic processes: accessing the metagenome as a resource of novel functional sequence space

P Lorenz, K Liebeton, F Niehaus, J Eck - Current opinion in biotechnology, 2002 - Elsevier
Historically, biotechnology has missed up to 99% of existing microbial resources by using
traditional screening techniques. Strategies of directly cloning 'environmental …

Strategies for accessing soil metagenome for desired applications

J Rajendhran, P Gunasekaran - Biotechnology advances, 2008 - Elsevier
Most of the microorganisms in nature are inaccessible as they are uncultivable in the
laboratory. Metagenomic approaches promise the accessibility of the genetic resources and …

Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples

G Lear, I Dickie, J Banks, S Boyer, HL Buckley… - New Zealand Journal of …, 2018 - JSTOR
Advances in the sequencing of DNA extracted from media such as soil and water offer huge
opportunities for biodiversity monitoring and assessment, particularly where the collection or …

Extraction of DNA from soil

P Robe, R Nalin, C Capellano, TM Vogel… - European Journal of Soil …, 2003 - Elsevier
There is an increased interest in the extraction of nucleic acids from various environmental
samples, since molecular techniques allow less biased access to a greater portion of …

Monitoring and source tracking of tetracycline resistance genes in lagoons and groundwater adjacent to swine production facilities over a 3-year period

S Koike, IG Krapac, HD Oliver… - Applied and …, 2007 - Am Soc Microbiol
To monitor the dissemination of resistance genes into the environment, we determined the
occurrence of tetracycline resistance (Tcr) genes in groundwater underlying two swine …

Nucleic acid purification using microfabricated silicon structures

NC Cady, S Stelick, CA Batt - Biosensors and Bioelectronics, 2003 - Elsevier
A microfluidic device has been designed, fabricated and tested for its ability to purify
bacteriophage lambda DNA and bacterial chromosomal DNA, a necessary prerequisite for …

Evaluation of extraction and purification methods for obtaining PCR-amplifiable DNA from compost for microbial community analysis

MG LaMontagne, FC Michel Jr, PA Holden… - Journal of …, 2002 - Elsevier
Analysis of microbial community structure in complex environmental samples using nucleic
acid techniques requires efficient unbiased DNA extraction procedures; however, humic …

Fungal communities from methane hydrate-bearing deep-sea marine sediments in South China Sea

X Lai, L Cao, H Tan, S Fang, Y Huang… - The ISME …, 2007 - academic.oup.com
To elucidate fungal diversity in methane hydrate-bearing deep-sea marine sediments in the
South China Sea, internal transcribed spacer (ITS) regions of rRNA genes from five different …

[PDF][PDF] Assessing PCR inhibition from humic substances

CD Matheson, C Gurney, N Esau… - The Open Enzyme …, 2010 - researchgate.net
Inhibition remains the greatest methodological challenge in molecular analysis of buried
biological remains. Inhibitory compounds associated with soil environments comprise …

Tetracycline residues and tetracycline resistance genes in groundwater impacted by swine production facilities

RI Mackie, S Koike, I Krapac, J Chee-Sanford… - Animal …, 2006 - Taylor & Francis
Antibiotics are used at therapeutic levels to treat disease; at slightly lower levels as
prophylactics; and at low, subtherapeutic levels for growth promotion and improvement of …