Hyperthermophilic enzymes: sources, uses, and molecular mechanisms for thermostability
C Vieille, GJ Zeikus - Microbiology and molecular biology reviews, 2001 - Am Soc Microbiol
Enzymes synthesized by hyperthermophiles (bacteria and archaea with optimal growth
temperatures of> 80° C), also called hyperthermophilic enzymes, are typically thermostable …
temperatures of> 80° C), also called hyperthermophilic enzymes, are typically thermostable …
Homing endonucleases: keeping the house in order
M Belfort, RJ Roberts - Nucleic acids research, 1997 - academic.oup.com
Homing endonucleases are rare-cutting enzymes encoded by introns and inteins. They
have striking structural and functional properties that distinguish them from restriction …
have striking structural and functional properties that distinguish them from restriction …
Complete Genome Sequence of the Methanogenic Archaeon, Methanococcus jannaschii
The complete 1.66-megabase pair genome sequence of an autotrophic archaeon,
Methanococcus jannaschii, and its 58-and 16-kilobase pair extrachromosomal elements …
Methanococcus jannaschii, and its 58-and 16-kilobase pair extrachromosomal elements …
Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
S Chong, FB Mersha, DG Comb, ME Scott, D Landry… - Gene, 1997 - Elsevier
A novel protein purification system has been developed which enables purification of free
recombinant proteins in a single chromatographic step. The system utilizes a modified …
recombinant proteins in a single chromatographic step. The system utilizes a modified …
High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5'to 3' …
FC Lawyer, S Stoffel, RK Saiki, SY Chang… - Genome …, 1993 - genome.cshlp.org
The Thermus aquaticus DNA polymerase I (Taq Pol I) gene was cloned into a plasmid
expression vector that utilizes the strong bacteriophage lambda PL promoter. A truncated …
expression vector that utilizes the strong bacteriophage lambda PL promoter. A truncated …
Method and product for localized or spatial detection of nucleic acid in a tissue sample
J Frisen, P Ståhl, J Lundeberg - US Patent 10,030,261, 2018 - Google Patents
The present invention relates to methods and products for the localized or spatial detection
of nucleic acid in a tissue sample and in particular to a method for localized detection of …
of nucleic acid in a tissue sample and in particular to a method for localized detection of …
[图书][B] Enzymes in the environment: activity, ecology, and applications
RG Burns, RP Dick - 2002 - taylorfrancis.com
Exploring the role of enzymes in biogeochemical processes, this reference explores the
function, molecular biology, and biochemistry of microorganisms and their intra-and extra …
function, molecular biology, and biochemistry of microorganisms and their intra-and extra …
Compilation, alignment, and phylogenetic relationships of DNA polymerases.
DK Braithwaite, J Ito - Nucleic acids research, 1993 - ncbi.nlm.nih.gov
SEQUENCE ALIGNMENT The multiple alignments ofthe amino acid sequences for this
update were performed in most cases by merely adding on to our original alignments (1) …
update were performed in most cases by merely adding on to our original alignments (1) …
Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature.
FB Perler, EO Davis, GE Dean, FS Gimble… - Nucleic acids …, 1994 - ncbi.nlm.nih.gov
INTRODUCTION Several archaeal, eubacterial and eucaryotic genes have been identified
with in-frame insertions that are excised at the protein level, not at the RNA level (1-13, 24 …
with in-frame insertions that are excised at the protein level, not at the RNA level (1-13, 24 …
Protein splicing and related forms of protein autoprocessing
H Paulus - Annual review of biochemistry, 2000 - annualreviews.org
▪ Abstract Protein splicing is a form of posttranslational processing that consists of the
excision of an intervening polypeptide sequence, the intein, from a protein, accompanied by …
excision of an intervening polypeptide sequence, the intein, from a protein, accompanied by …