In this article we explain how current events in the field of phage therapy may positively
influence its future development. We discuss the shift in position of the authorities …

Much of our current understanding of microbiology is based on the application of genetic
engineering procedures. Since their inception (more than 30 years ago), methods based …

Allelic exchange is an efficient method of bacterial genome engineering. This protocol
describes the use of this technique to make gene knockouts and knock-ins, as well as single …

Within recent years, it has been established that extracellular DNA is a key constituent of the
matrix of microbial biofilms. In addition, it has recently been demonstrated that DNA binds …

Dietary intake of l-carnitine can promote cardiovascular diseases in humans through
microbial production of trimethylamine (TMA) and its subsequent oxidation to trimethylamine …

Bacteria possess a diverse range of mechanisms for inhibiting competitors, including
bacteriocins, tailocins, type VI secretion systems and contact-dependent inhibition (CDI) …

Bacteria living as biofilm are frequently reported to exhibit inherent tolerance to antimicrobial
compounds, and might therefore contribute to the persistence of infections. Antimicrobial …

When grown as a biofilm in laboratory flow chambers Pseudomonas aeruginosa can
develop mushroom‐shaped multicellular structures consisting of distinct subpopulations in …

Bacteria growing in biofilms are physiologically heterogeneous, due in part to their
adaptation to local environmental conditions. Here, we characterized the local transcriptome …

Mature Pseudomonas aeruginosa biofilms undergo specific developmental events. Using a
bacteriophage mutant, generated by deletion of the entire filamentous Pf4 prophage, we …