Techniques for the analysis of protein-protein interactions in vivo

S Xing, N Wallmeroth, KW Berendzen… - Plant physiology, 2016 - academic.oup.com
Identifying key players and their interactions is fundamental for understanding biochemical
mechanisms at the molecular level. The ever-increasing number of alternative ways to …

Diversity in Genetic In Vivo Methods for Protein-Protein Interaction Studies: from the Yeast Two-Hybrid System to the Mammalian Split-Luciferase System

B Stynen, H Tournu, J Tavernier… - … and molecular biology …, 2012 - Am Soc Microbiol
The yeast two-hybrid system pioneered the field of in vivo protein-protein interaction
methods and undisputedly gave rise to a palette of ingenious techniques that are constantly …

Architecture of chloroplast TOC–TIC translocon supercomplex

H Liu, A Li, JD Rochaix, Z Liu - Nature, 2023 - nature.com
Chloroplasts rely on the translocon complexes in the outer and inner envelope membranes
(the TOC and TIC complexes, respectively) to import thousands of different nuclear-encoded …

[HTML][HTML] The chloroplast protein import system: from algae to trees

LX Shi, SM Theg - Biochimica et Biophysica Acta (BBA)-Molecular Cell …, 2013 - Elsevier
Chloroplasts are essential organelles in the cells of plants and algae. The functions of these
specialized plastids are largely dependent on the~ 3000 proteins residing in the organelle …

What do the sun and the sky tell us about the camera?

JF Lalonde, SG Narasimhan, AA Efros - International Journal of Computer …, 2010 - Springer
As the main observed illuminant outdoors, the sky is a rich source of information about the
scene. However, it is yet to be fully explored in computer vision because its appearance in …

New insights into the mechanism of chloroplast protein import and its integration with protein quality control, organelle biogenesis and development

YD Paila, LGL Richardson, DJ Schnell - Journal of molecular biology, 2015 - Elsevier
The translocons at the outer (TOC) and the inner (TIC) envelope membranes of chloroplasts
mediate the targeting and import of several thousand nucleus-encoded preproteins that are …

The similarity between N-terminal targeting signals for protein import into different organelles and its evolutionary relevance

M Kunze, J Berger - Frontiers in physiology, 2015 - frontiersin.org
The proper distribution of proteins between the cytosol and various membrane-bound
compartments is crucial for the functionality of eukaryotic cells. This requires the cooperation …

A split GAL4 RUBY assay for visual in planta detection of protein–protein interactions

J Chen, M Luo, P Hands, V Rolland, J Zhang… - The Plant …, 2023 - Wiley Online Library
Protein–protein interactions (PPIs) are a fundamental process in cellular biogenesis. Here
we have developed a split GAL4 RUBY assay that enables macroscopically visual PPI …

Novel insights into the contribution of plastoglobules and reactive oxygen species to chromoplast differentiation

L Morelli, S Torres‐Montilla, G Glauser… - New …, 2023 - Wiley Online Library
Plant tissues can be enriched in phytonutrients not only by stimulating their biosynthesis but
also by providing appropriate sink structures for their sequestering and storage. In the case …

Systems analysis of plant functional, transcriptional, physical interaction, and metabolic networks

GW Bassel, A Gaudinier, SM Brady, L Hennig… - The Plant …, 2012 - academic.oup.com
Physiological responses, developmental programs, and cellular functions rely on complex
networks of interactions at different levels and scales. Systems biology brings together high …