Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions
RC Rodrigues, JJ Virgen-Ortíz, JCS Dos Santos… - Biotechnology …, 2019 - Elsevier
Lipases are the most widely used enzymes in biocatalysis, and the most utilized method for
enzyme immobilization is using hydrophobic supports at low ionic strength. This method …
enzyme immobilization is using hydrophobic supports at low ionic strength. This method …
On the taught new tricks of enzymes immobilization: An all-inclusive overview
The primary means of immobilizing enzymes are to boost the enzyme productivity and
operational stability, alongside facilitating the reuse of enzymes. Notwithstanding the …
operational stability, alongside facilitating the reuse of enzymes. Notwithstanding the …
[HTML][HTML] Agarose and its derivatives as supports for enzyme immobilization
Agarose is a polysaccharide obtained from some seaweeds, with a quite particular structure
that allows spontaneous gelation. Agarose-based beads are highly porous, mechanically …
that allows spontaneous gelation. Agarose-based beads are highly porous, mechanically …
Smart chemistry of enzyme immobilization using various support matrices–a review
The surface chemistry, pendent functional entities, and ease in tunability of various materials
play a central role in properly coordinating with enzymes for immobilization purposes. Due …
play a central role in properly coordinating with enzymes for immobilization purposes. Due …
“Smart” chemistry and its application in peroxidase immobilization using different support materials
In the past few decades, the enzyme immobilization technology has been exploited a lot and
thus became a matter of rational design. Immobilization is an alternative approach to bio …
thus became a matter of rational design. Immobilization is an alternative approach to bio …
Protein immobilization strategies for protein biochips
In the past few years, protein biochips have emerged as promising proteomic and diagnostic
tools for obtaining information about protein functions and interactions. Important …
tools for obtaining information about protein functions and interactions. Important …
Immobilized enzymes: methods and applications
W Tischer, F Wedekind - Biocatalysis-from discovery to application, 1999 - Springer
Immobilized enzymes are used in organic syntheses to fully exploit the technical and
economical advantages of biocatalysts based on isolated enzymes. Immobilization enables …
economical advantages of biocatalysts based on isolated enzymes. Immobilization enables …
Chemical strategies for generating protein biochips
P Jonkheijm, D Weinrich, H Schröder… - Angewandte Chemie …, 2008 - Wiley Online Library
Protein biochips are at the heart of many medical and bioanalytical applications. Increasing
interest has been focused on surface activation and subsequent functionalization strategies …
interest has been focused on surface activation and subsequent functionalization strategies …
Co‐immobilized phosphorylated cofactors and enzymes as self‐sufficient heterogeneous biocatalysts for chemical processes
S Velasco‐Lozano, AI Benítez‐Mateos… - Angewandte Chemie …, 2017 - Wiley Online Library
Enzyme cofactors play a major role in biocatalysis, as many enzymes require them to
catalyze highly valuable reactions in organic synthesis. However, the cofactor recycling is …
catalyze highly valuable reactions in organic synthesis. However, the cofactor recycling is …
A single step purification, immobilization, and hyperactivation of lipases via interfacial adsorption on strongly hydrophobic supports
A Bastida, P Sabuquillo, P Armisen… - Biotechnology and …, 1998 - Wiley Online Library
A number of bacterial lipases can be immobilized in a rapid and strong fashion on octyl‐
agarose gels (eg, lipases from Candida antarctica, Pseudomonas fluorescens, Rhizomucor …
agarose gels (eg, lipases from Candida antarctica, Pseudomonas fluorescens, Rhizomucor …