Non-coding RNAs: Epigenetic regulators of bone development and homeostasis

MQ Hassan, CE Tye, GS Stein, JB Lian - Bone, 2015 - Elsevier
Non-coding RNAs (ncRNAs) have evolved in eukaryotes as epigenetic regulators of gene
expression. The most abundant regulatory ncRNAs are the 20–24 nt small microRNAs …

smiFISH and FISH-quant–a flexible single RNA detection approach with super-resolution capability

N Tsanov, A Samacoits, R Chouaib… - Nucleic acids …, 2016 - academic.oup.com
Single molecule FISH (smFISH) allows studying transcription and RNA localization by
imaging individual mRNAs in single cells. We present smiFISH (single molecule …

[HTML][HTML] microRNA-guided diagnostics in clinical samples

D Gustafson, K Tyryshkin, N Renwick - Best practice & research Clinical …, 2016 - Elsevier
miRNA-guided diagnostics is a powerful molecular approach for evaluating clinical samples
through miRNA detection and/or visualization. To date, this approach has been successfully …

Long non-coding RNA C2dat1 regulates CaMKIIδ expression to promote neuronal survival through the NF-κB signaling pathway following cerebral ischemia

Q Xu, F Deng, Z Xing, Z Wu, B Cen, S Xu, Z Zhao… - Cell death & …, 2016 - nature.com
Increasing evidence has demonstrated a significant role of long non-coding RNAs
(lncRNAs) in diverse biological processes. However, their functions in cerebral ischemia …

[HTML][HTML] Promoter and enhancer RNAs regulate chromatin reorganization and activation of miR-10b/HOXD locus, and neoplastic transformation in glioma

E Deforzh, EJ Uhlmann, E Das, A Galitsyna, R Arora… - Molecular cell, 2022 - cell.com
Summary miR-10b is silenced in normal neuroglial cells of the brain but commonly activated
in glioma, where it assumes an essential tumor-promoting role. We demonstrate that the …

Full-length mRNA sequencing uncovers a widespread coupling between transcription initiation and mRNA processing

SY Anvar, G Allard, E Tseng, GM Sheynkman… - Genome biology, 2018 - Springer
Background The multifaceted control of gene expression requires tight coordination of
regulatory mechanisms at transcriptional and post-transcriptional level. Here, we studied the …

MALAT1: a regulator of inflammatory cytokines in diabetic complications

AD Gordon, S Biswas, B Feng… - … , diabetes & metabolism, 2018 - Wiley Online Library
Summary Objectives and Design In this study, we examined the role of MALAT 1, a highly
conserved nuclear long non‐coding RNA molecule, in chronic diabetic complications …

[HTML][HTML] Single molecule fluorescence in situ hybridisation for quantitating post-transcriptional regulation in Drosophila brains

L Yang, J Titlow, D Ennis, C Smith, J Mitchell, FL Young… - Methods, 2017 - Elsevier
RNA in situ hybridization is a powerful method to investigate post-transcriptional regulation,
but analysis of intracellular mRNA distributions in thick, complex tissues like the brain poses …

Reference genes for quantitative Arabidopsis single molecule RNA fluorescence in situ hybridization

S Duncan, HE Johansson, Y Ding - Journal of Experimental …, 2023 - academic.oup.com
Subcellular mRNA quantities and spatial distributions are fundamental for driving gene
regulatory programmes. Single molecule RNA fluorescence in situ hybridization (smFISH) …

Stellaris® RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells

AV Orjalo, HE Johansson - Long Non-Coding RNAs: Methods and …, 2016 - Springer
RNA fluorescence in situ hybridization (FISH), long an indispensable tool for the detection
and localization of RNA, is becoming an increasingly important complement to other gene …