Golden Mutagenesis: An efficient multi-site-saturation mutagenesis approach by Golden Gate cloning with automated primer design

P Püllmann, C Ulpinnis, S Marillonnet, R Gruetzner… - Scientific reports, 2019 - nature.com
Site-directed methods for the generation of genetic diversity are essential tools in the field of
directed enzyme evolution. The Golden Gate cloning technique has been proven to be an …

Iron-Catalyzed Linear Trimerization of Phenylacetylenes for the Formation of Conjugated Dienynes

JL Wright-Keely - 2020 - search.proquest.com
A tert-butyl-substituted bis-pyrrolide pincer ligand, 2, 6-bis (3, 5-di-tert-butylpyrrol-2-yl)
pyridine (L tButyl, tButyl), was synthesized and complexed to iron to form the ether complex …