Past, present and future of ICSI in livestock species

O Briski, DF Salamone - Animal Reproduction Science, 2022 - Elsevier
During the past 2 decades, intracytoplasmic sperm injection (ICSI) has become a routine
technique for clinical applications in humans. The widespread use among domestic species …

Intracytoplasmic sperm injection in domestic and wild mammals

DF Salamone, NG Canel, MB Rodríguez - Reproduction, 2017 - rep.bioscientifica.com
Intracytoplasmic sperm injection (ICSI) has become a useful technique for clinical
applications in the horse-breeding industry. However, both ICSI blastocyst and offspring …

Sperm selection by thermotaxis improves ICSI outcome in mice

S Pérez-Cerezales, R Laguna-Barraza… - Scientific Reports, 2018 - nature.com
The ejaculate is a heterogeneous pool of spermatozoa containing only a small
physiologically adequate subpopulation for fertilization. As there is no method to isolate this …

Phospholipase C Zeta 1 (PLCZ1): the function and potential for fertility assessment and in vitro embryo production in cattle and horses

RA Gonzalez-Castro, EM Carnevale - Veterinary Sciences, 2023 - mdpi.com
Simple Summary After sperm–oocyte fusion, oocytes are activated by a sperm factor for
fertilization and embryo development success. Evidence proposes that sperm-borne …

Bovine ICSI: Limiting factors, strategies to improve its efficiency and alternative approaches

F Fuentes, E Munoz, MJ Contreras, ME Arias, R Felmer - Zygote, 2022 - cambridge.org
Intracytoplasmic sperm injection (ICSI) is an assisted reproductive technique mainly used to
overcome severe infertility problems associated with the male factor, but in cattle its …

Lower blastocyst quality after conventional vs. Piezo ICSI in the horse reflects delayed sperm component remodeling and oocyte activation

RM Salgado, JG Brom-de-Luna, HL Resende… - Journal of assisted …, 2018 - Springer
Purpose The aim of this study was to evaluate the differential effects of conventional and
Piezo-driven ICSI on blastocyst development, and on sperm component remodeling and …

Intracytoplasmic sperm injection in cattle

V Unnikrishnan, J Kastelic, J Thundathil - Genes, 2021 - mdpi.com
Intracytoplasmic sperm injection (ICSI) involves the microinjection of sperm into a matured
oocyte. Although this reproductive technology is successfully used in humans and many …

Adverse effects of single neutrophil extracellular trap-derived components on bovine sperm function

C Moya, R Rivera-Concha, F Pezo, P Uribe, M Schulz… - Animals, 2022 - mdpi.com
Simple Summary Neutrophil extracellular traps (NETs) play a key role in fertilisation by
eliminating microorganisms and entrapping spermatozoa in the female reproductive tract …

Significance of incorporating measures of sperm production and function into rat toxicology studies

SD Perreault, AM Cancel - Reproduction, 2001 - rep.bioscientifica.com
Studies have shown inhibitory effects of endogenous opioids on LH secretion in early post-
natal heifers. However, it is not clear whether these effects change during the rest of the …

Defective sperm head decondensation undermines the success of ICSI in the bovine

L Águila, R Felmer, ME Arias, F Navarrete… - …, 2017 - rep.bioscientifica.com
The efficiency of intracytoplasmic sperm injection (ICSI) in the bovine is low compared to
other species. It is unknown whether defective oocyte activation and/or sperm head …