Dimethyl sulfoxide: a central player since the dawn of cryobiology, is efficacy balanced by toxicity?

M Awan, I Buriak, R Fleck, B Fuller, A Goltsev… - Regenerative …, 2020 - Taylor & Francis
Dimethyl sulfoxide (DMSO) is the cryoprotectant of choice for most animal cell systems since
the early history of cryopreservation. It has been used for decades in many thousands of cell …

Dimethyl sulfoxide-free cryopreservation for cell therapy: A review

L Weng, PR Beauchesne - Cryobiology, 2020 - Elsevier
Cell-based therapeutics promise to transform the treatment of a wide range of diseases
including cancer, genetic and degenerative disorders, or severe injuries. Many of the …

Advances in the slow freezing cryopreservation of microencapsulated cells

H Gurruchaga, LS Del Burgo, RM Hernandez… - Journal of controlled …, 2018 - Elsevier
Over the past few decades, the use of cell microencapsulation technology has been
promoted for a wide range of applications as sustained drug delivery systems or as cells …

Cryopreservation of NK and T cells without DMSO for adoptive cell-based immunotherapy

X Yao, S Matosevic - BioDrugs, 2021 - Springer
Dimethylsufoxide (DMSO) being universally used as a cryoprotectant in clinical adoptive cell-
therapy settings to treat hematological malignancies and solid tumors is a growing concern …

Protective roles of mesenchymal stem cells on skin photoaging: a narrative review

J Lv, S Yang, M Lv, J Lv, Y Sui, S Guo - Tissue and Cell, 2022 - Elsevier
Skin is a natural barrier of human body and a visual indicator of aging process. Exposure to
ultraviolet (UV) radiation in the sunlight may injure the skin tissues and cause local damage …

Cryostorage of mesenchymal stem cells and biomedical cell-based products

DD Linkova, YP Rubtsova, MN Egorikhina - Cells, 2022 - mdpi.com
Mesenchymal stem cells (MSCs) manifest vast opportunities for clinical use due both to their
ability for self-renewal and for effecting paracrine therapeutic benefits. At the same time …

Novel Cryopreservation Approach Providing Off‐the‐Shelf Availability of Human Multipotent Mesenchymal Stromal Cells for Clinical Applications

O Rogulska, O Tykhvynska, O Revenko… - Stem Cells …, 2019 - Wiley Online Library
Cryopreservation is the only established method to provide long‐term storage and fast
availability of cellular product for therapeutic applications. The overwhelming majority of …

Cryostructuring of polymer systems. 47. Preparation of wide porous gelatin-based cryostructurates in sterilizing organic media and assessment of the suitability of thus …

VI Lozinsky, VK Kulakova, RV Ivanov, AY Petrenko… - e-Polymers, 2018 - degruyter.com
New gelatin-based cryostructurates have been elaborated and tested as scaffolds for three-
dimensional (3D) cell culturing. Scaffold preparation included dissolution of Type A gelatin …

Cryopreservation with DMSO affects the DNA integrity, apoptosis, cell cycle and function of human bone mesenchymal stem cells

Y Ding, S Liu, J Liu, S Jin, J Wang - Cryobiology, 2024 - Elsevier
Cryopreservation (CP) enables pooling and long-term banking of various types of cells,
which is indispensable for the cell therapeutics. Dimethyl sulfoxide (DMSO) is universally …

Strategies in developing dimethyl sulfoxide (DMSO)-free cryopreservation protocols for biotherapeutics

MD Ekpo, GF Boafo, J Xie, X Liu, C Chen… - Frontiers in …, 2022 - frontiersin.org
Cryopreservation is a technology employed in long-term storage of biologics achieved by
cooling to cryogenic temperatures (1, 2). This preservation technique has become …