The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert
DNA cassettes into eukaryotic chromosomes is widespread (1–9). Indeed, a PubMed search …

DNA supercoiling is a major regulator of transcription in bacteria. Negative supercoiling acts
both by promoting the formation of nucleoprotein structures containing wrapped DNA and by …

We have explored the Escherichia coli chromosome architecture by genetic dissection,
using a site‐specific recombination system that reveals the spatial proximity of distant DNA …

We show that positively supercoiled [(+) SC] DNA is the preferred substrate for Escherichia
coli topoisomerase IV (topo IV). We measured topo IV relaxation of (−) and (+) supercoils in …

The crystal structure of a Flp recombinase tetramer bound to a Holliday junction intermediate
has been determined at 2.65 Å resolution. Only one of Flp's two domains, containing the …

Site-specific DNA recombination is important for basic cellular functions including viral
integration, control of gene expression, production of genetic diversity and segregation of …

We study here global and local entanglements of open protein chains by implementing the
concept of knotoids. Knotoids have been introduced in 2012 by Vladimir Turaev as a …

We report on a computational study of the statics and dynamics of long flexible linear
polymers that spontaneously knot and unknot. Specifically, the equilibrium self …

The λ site‐specific recombination pathway has enjoyed the sequential attentions of
geneticists, biochemists, and structural biologists for more than 50 years. It has proven to be …

Reconnection is a fundamental event in many areas of science, from the interaction of
vortices in classical and quantum fluids and magnetic flux tubes in magnetohydrodynamics …