Although at least 35,000 human genes have been sequenced and mapped, adequate
expression or functional information is available for only∼ 15% of them. Gene-trap …

Publisher Summary There are now several different methods for ectopic expression in
Drosophila, each with its merits and its limitations. The first technique is to drive expression …

Fruit flies of the genus Drosophila have been an attractive and effective genetic model
organism since Thomas Hunt Morgan and colleagues made seminal discoveries with them …

We generated a library of~ 1000 Drosophila stocks in which we inserted a construct in the
intron of genes allowing expression of GAL4 under control of endogenous promoters while …

We have designed a system for targeted gene expression that allows the selective activation
of any cloned gene in a wide variety of tissueand cell-specific patterns. The gene encoding …

The Drosophila gene eyeless (ey) encodes a transcription factor with both a paired domain
and a homeodomain. It is homologous to the mouse Small eye (Pax-6) gene and to the …

A general strategy for selecting insertion mutations in mice has been devised. Constructs
lacking a promoter and including a beta-galactosidase gene, or a reporter gene encoding a …

Germ-line stem cells (GSCs) serve as the source for gametogenesis in diverse organisms.
We cloned and characterized the Drosophila piwi gene and showed that it is required for the …

In Drosophila, enhancer trap strategies allow rapid access to expression patterns, molecular
data, and mutations in trapped genes. However, they do not give any information at the …

Abstract The Berkeley Drosophila Genome Project (BDGP) strives to disrupt each
Drosophila gene by the insertion of a single transposable element. As part of this effort …