Although animal experiments are indispensable for preclinical screening in the drug
discovery process, various issues such as ethical considerations and species differences …

This review encompasses the most important advances in liver functions and hepatotoxicity
and analyzes which mechanisms can be studied in vitro. In a complex architecture of nested …

Background Conventional preclinical models often miss drug toxicities, meaning the harm
these drugs pose to humans is only realized in clinical trials or when they make it to market …

Liver biology and function, drug-induced liver injury (DILI) and liver diseases are difficult to
study using current in vitro models such as primary human hepatocyte (PHH) monolayer …

Acetaminophen (APAP) is one of the most widely used drugs. Though safe at therapeutic
doses, overdose causes mitochondrial dysfunction and centrilobular necrosis in the liver …

Drug-induced liver injury (DILI) continues to be a major source of clinical attrition,
precautionary warnings, and post-market withdrawal of drugs. Accordingly, there is a need …

Hepatoma cell lines are frequently used as in vitro alternatives to primary human
hepatocytes. Cell lines are characterized by their unlimited life span, stable phenotype, high …

Cytotoxicity assays were among the first in vitro bioassay methods used to predict toxicity of
substances to various tissues. In vitro cytotoxicity testing provides a crucial means for safety …

Cultures of primary hepatocytes and hepatoma cell line HepG2 are frequently used in in
vitro models for human biotransformation studies. In this study, we characterized and …

The performance of alamar blue and 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyl tetrazolium
bromide (MTT) cell viability assays in a high through-put format were compared. A total of …