Delivery of proteins to the vacuole of the yeast Saccharomyces cerevisiae provides an
excellent model system in which to study vacuole and lysosome biogenesis and membrane …

Molecules involved in late steps of neurotransmitter release at the synapse can be
examined by noting the two speeds of the components of the exocytotic burst that are …

S. cerevisiae inherits its vacuole by projecting vacuole-derived membrane vesicles and
tubules into the bud, where they fuse to establish the daughter vacuole. This homotypic …

Small molecules that target specific DNA sequences have the potential to control gene
expression. Ligands designed for therapeutic application must bind any predetermined DNA …

The basic reaction mechanisms for membrane fusion in the trafficking of intracellular
membranes and in exocytosis are probably identical. But in contrast to regulated exocytosis …

Membrane fusion is necessary both in the eukaryotic secretory pathway and for the
inheritance of organelles during the cell cycle. In the secretory pathway, heterotypic fusion …

Every membrane-bordered organelle in a particular cell type has a characteristic copy
number, size, and position, which reflect its cellular function. Mitochondria, for Partitioning …

The homotypic fusion of yeast vacuoles includes a 'docking'step, which we show here to
consist of two sequential reactions: a reversible 'tethering'mediated by the GTPase Ypt7, and …

In the budding yeast Saccharomyces cerevisiae, vacuoles are inherited by the projection of
vesicles and tubules from the mother‐cell vacuole into the growing daughter cell during the …

Eukaryotic cells have the ability to degrade proteins and organelles by selective and
nonselective modes of micro-and macroautophagy. In addition, there exist both constitutive …