Amphipols (APols) are short amphipathic polymers that can substitute for detergents at the
transmembrane surface of membrane proteins (MPs) and, thereby, keep them soluble in …

Structural studies of G protein-coupled receptors (GPCRs) gave insights into molecular
mechanisms of their action and contributed significantly to molecular pharmacology. This is …

Production of integral membrane proteins (IMPs) in a folded state is a key prerequisite for
their functional and structural studies. In cell-free (CF) expression systems membrane …

Cell-free protein production has become a core technology in the rapidly spreading field of
synthetic biology. In particular the synthesis of membrane proteins, highly problematic …

Simple Summary Membrane proteins are essential to all forms of life. Millions of membrane
proteins are found in the lipid membrane layer that surrounds cells, and in the lipid …

Nonionic amphipols (NAPols) synthesized by homotelomerization of an amphiphatic
monomer are able to keep membrane proteins (MPs) stable and functional in the absence of …

Cell-free protein production opens new perspectives for the direct manipulation of
expression compartments in combination with reduced complexity of physiological …

Although nearly half of today's major pharmaceutical drugs target human integral membrane
proteins (hIMPs), only 30 hIMP structures are currently available in the Protein Data Bank …

High amounts of membrane protein samples are needed for structural or functional analysis
and a first bottleneck is often to obtain sufficient production efficiencies. The reduced …

A challenging requirement for X-ray crystallography or NMR structure determination of
membrane proteins (MPs), in contrast to soluble proteins, is the necessary use of …