Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …

Genome editing technologies hold great promise for numerous applications including the
understanding of cellular and disease mechanisms and the development of gene and …

CRISPR-based genome editing holds promise for genome engineering and other
applications in diverse organisms. Defining and improving the genome-wide and …

Adenine base editors (ABE) are genome-editing tools that have been harnessed to
introduce precise A• T to G• C conversion. However, the low activity of ABE at certain sites …

Prime editing technologies enable precise genome editing without the caveats of CRISPR
nuclease-based methods. Nonetheless, current approaches to identify and isolate prime …

Deaminase fused-Cas9 base editing technologies have enabled precise single-nucleotide
genomic editing without the need for the introduction of damaging double-stranded breaks …

In polycystic kidney disease (PKD), microscopic tubules expand into macroscopic cysts.
Among the world's most common genetic disorders, PKD is inherited via heterozygous loss …

Isogenic induced pluripotent stem cell (iPSC) lines are currently mostly created by homology
directed repair evoked by a double-strand break (DSB) generated by CRISPR-Cas9 …

To expand the scope of genomic editing, a C-to-G transversion-based editor called CGBE
has been developed for precise single-nucleotide genomic editing. However, limited editing …

CRISPR-Cas9 and novel cas fusion proteins leveraging specific DNA targeting ability
combined with deaminases or reverse transcriptases have revolutionized genome editing …