Correlative microscopy, the integration of two or more microscopy techniques performed on
the same sample, produces results that emphasize the strengths of each technique while …

We review the emerging method of super-resolved cryogenic correlative light and electron
microscopy (srCryoCLEM). Super-resolution (SR) fluorescence microscopy and cryogenic …

The optical microscope is one of the oldest scientific instruments that is still used in forefront
research. Ernst Abbe's nineteenth century formulation of the resolution limit in microscopy let …

As in other imaging methods based on waves, the resolution of ultrasound imaging is limited
by the wavelength. However, the diffraction-limit can be overcome by super-localizing single …

Correlative light and electron microscopy (CLEM) has become a powerful tool in life
sciences. Particularly cryo‐CLEM, the combination of fluorescence cryo‐microscopy (cryo …

G protein–coupled receptors (GPCRs), including dopamine receptors, represent a group of
important pharmacological targets. An increased formation of dopamine receptor D2 …

We introduce a super-resolution technique for fluorescence cryo-microscopy based on
photoswitching of standard genetically encoded fluorescent marker proteins in intact …

Highlights•CryoFM allows imaging of vitrified biological samples with fluorescence
microscopy.•There are significant challenges to achieve high-resolution cryoFM …

Abstract We introduce Cryogenic Optical Localization in 3D (COLD), a method to localize
multiple fluorescent sites within a single small protein with Angstrom resolution. We …

We present a bimodal endocytic tracer, fluorescent BSA-gold (fBSA-Au), as a fiducial marker
for 2D and 3D correlative light and electron microscopy (CLEM) applications. fBSA-Au …