Amination of enzymes to improve biocatalyst performance: Coupling genetic modification and physicochemical tools

RC Rodrigues, O Barbosa, C Ortiz… - RSC …, 2014 - pubs.rsc.org
Improvement of the features of an enzyme is in many instances a pre-requisite for the
industrial implementation of these exceedingly interesting biocatalysts. To reach this goal …

Chemical recycling of polyhydroxyalkanoates as a method towards sustainable development

H Ariffin, H Nishida, MA Hassan… - Biotechnology journal, 2010 - Wiley Online Library
Chemical recycling of bio‐based polymers polyhydroxyalkanoates (PHAs) by thermal
degradation was investigated from the viewpoint of biorefinery. The thermal degradation …

Liquid droplet of protein-polyelectrolyte complex for high-concentration formulations

A Matsuda, M Mimura, T Maruyama… - Journal of …, 2018 - Elsevier
The formulation of high-concentration protein solutions is a challenging issue for achieving
subcutaneous administration. Previously, we developed a method of precipitation …

Protein thermal stability: The role of protein structure and aqueous environment

E Pechkova, V Sivozhelezov, C Nicolini - Archives of biochemistry and …, 2007 - Elsevier
A comprehensive bioinformatic analysis was performed on all protein homologous pairs
from mesophilic and thermophilic microorganisms present in the RCSB Protein Data Bank in …

Production of alpha-amylase from Aspergillus oryzae for several industrial applications in a single step

MC Porfirif, EJ Milatich, BM Farruggia… - … of Chromatography B, 2016 - Elsevier
A one-step method as a strategy of alpha-amylase concentration and purification was
developed in this work. This methodology requires the use of a very low concentration of …

Chymotrypsin–poly vinyl sulfonate interaction studied by dynamic light scattering and turbidimetric approaches

V Boeris, D Spelzini, JP Salgado, G Picó… - … et Biophysica Acta (BBA …, 2008 - Elsevier
The formation of non-soluble complexes between a positively charged protein and a strong
anionic polyelectrolyte, chymotrypsin, and poly vinyl sulfonate, respectively, was studied …

Protein-flexible chain polymer interactions to explain protein partition in aqueous two-phase systems and the protein–polyelectrolyte complex formation

V Boeris, B Farruggia, B Nerli, D Romanini… - International journal of …, 2007 - Elsevier
Complexes formation between two model proteins (catalase and chymotrypsin) and
polyelectrolytes (polyvinyl sulphonate and polyacrilic acid) and a non-charged flexible chain …

Enzyme switch by complementary polymer pair system (CPPS)

S Tomita, L Ito, H Yamaguchi, G Konishi, Y Nagasaki… - Soft Matter, 2010 - pubs.rsc.org
Manipulation of enzyme activity at will presents various research and industrial applications.
Herein we describe development of a technology for inactivation and reactivation of enzyme …

Bioseparation of papain from Carica papaya latex by precipitation of papain–poly (vinyl sulfonate) complexes

M Braia, M Ferrero, MV Rocha, D Loureiro… - Protein expression and …, 2013 - Elsevier
The formation of insoluble complexes between enzymes and polyelectrolytes is a suitable
technique for isolating these biomolecules from natural sources, because it is a simple and …

Precipitation with poly acrylic acid as a trypsin bioseparation strategy

MC Porfiri, M Braia, B Farruggia, G Pico… - Process Biochemistry, 2009 - Elsevier
Precipitation of enzymes with reversible soluble–insoluble polymers is a simple approach
which can be easily scaled up. This work reports investigations aiming at verifying the …