Essential genes encode the processes that are necessary for life. Until recently, commonly
applied binary classifications left no space between essential and non-essential genes. In …

Translation is the fundamental process of protein synthesis and is catalysed by the ribosome
in all living cells. Here we use advances in cryo-electron tomography and sub-tomogram …

Background Genome-wide functional genetic studies of food-borne pathogens could
provide a comprehensive understanding of the mechanisms underline their survival and …

The development of advanced genetic tools is boosting microbial engineering which can
potentially tackle wide-ranging challenges currently faced by our society. Here we present …

With the Internet's rapid development and the increasing amount of netizens, social
contradictions frequently manifest over the Internet. Public emergencies develop and spread …

Identifying open reading frames (ORFs) being translated is not a trivial task. ProTInSeq is a
technique designed to characterize proteomes by sequencing transposon insertions …

Tn5 transposase, which can efficiently tagment the genome, has been widely adopted as a
molecular tool in next-generation sequencing, from short-read sequencing to more complex …

A non-pathogenic Mycoplasma pneumoniae-based chassis is leading the development of
live biotherapeutic products (LBPs) for respiratory diseases. However, reports connecting …

A first clue to gene function can be obtained by examining whether a gene is required for life
in certain standard conditions, that is, whether a gene is essential. In bacteria, essential …

The removal of unwanted genetic material is a key aspect in many synthetic biology efforts
and often requires preliminary knowledge of which genomic regions are dispensable …