The proteins extracellular signal-regulated kinase 1 (ERK1) and ERK2 are the downstream
components of a phosphorelay pathway that conveys growth and mitogenic signals largely …

Extracellular signal-regulated kinase (ERK) has long been studied as a key driver of both
essential cellular processes and disease. A persistent question has been how this single …

Cell death events continuously challenge epithelial barrier function yet are crucial to
eliminate old or critically damaged cells. How such apoptotic events are spatio-temporally …

Genetically encoded fluorescent biosensors are powerful tools for monitoring biochemical
activities in live cells, but their multiplexing capacity is limited by the available spectral …

ERK signaling regulates the expression of target genes, but it is unclear how ERK activity
dynamics are interpreted. Here, we investigate this question using simultaneous, live, single …

Intratumoral heterogeneity is associated with aggressive tumor behavior, therapy resistance,
and poor patient outcomes. Such heterogeneity is thought to be dynamic, shifting over …

The Ras-ERK signaling pathway regulates diverse cellular processes in response to
environmental stimuli and contains important therapeutic targets for cancer. Recent single …

Activating mutations in RAS are present in~ 30% of human tumors, and the resulting
aberrations in ERK/MAPK signaling play a central role in oncogenesis. However, the form of …

Cell-to-cell heterogeneity in metabolism plays an unknown role in physiology and
pharmacology. To functionally characterize cellular variability in metabolism, we treated …

Cancer cells have heterogeneous fitness, and this heterogeneity stems from genetic and
epigenetic sources. Here, we sought to assess the contribution of asymmetric mitosis (AM) …