Quantitative phase imaging: recent advances and expanding potential in biomedicine

TL Nguyen, S Pradeep, RL Judson-Torres, J Reed… - ACS …, 2022 - ACS Publications
Quantitative phase imaging (QPI) is a label-free, wide-field microscopy approach with
significant opportunities for biomedical applications. QPI uses the natural phase shift of light …

[HTML][HTML] Considerations and challenges in studying liquid-liquid phase separation and biomolecular condensates

S Alberti, A Gladfelter, T Mittag - Cell, 2019 - cell.com
Evidence is now mounting that liquid-liquid phase separation (LLPS) underlies the formation
of membraneless compartments in cells. This realization has motivated major efforts to …

Photonic artifacts in ratiometric luminescence nanothermometry

SJW Vonk, TP van Swieten, A Cocina, FT Rabouw - Nano Letters, 2023 - ACS Publications
Ongoing developments in science and technology require temperature measurements at
increasingly higher spatial resolutions. Nanocrystals with temperature-sensitive …

In-line holographic microscopy with model-based analysis

C Martin, LE Altman, S Rawat, A Wang… - Nature Reviews …, 2022 - nature.com
An in-line holographic microscope is an optical microscope outfitted with a coherent light
source, such as a laser. Light scattered by the specimen interferes with the transmitted …

[HTML][HTML] Fluorescence microscopy—an outline of hardware, biological handling, and fluorophore considerations

SM Hickey, B Ung, C Bader, R Brooks, J Lazniewska… - Cells, 2021 - mdpi.com
Fluorescence microscopy has become a critical tool for researchers to understand biological
processes at the cellular level. Micrographs from fixed and live-cell imaging procedures …

Cell refractive index: Models, insights, applications and future perspectives

B Gul, S Ashraf, S Khan, H Nisar, I Ahmad - … and photodynamic therapy, 2021 - Elsevier
Cell refractive index (RI) is an intrinsic optical parameter that governs the propagation of
light (ie, scattering and absorption) in the cell matrix. The RI of cell is sensitively correlated …

Three‐dimensional correlative single‐cell imaging utilizing fluorescence and refractive index tomography

M Schürmann, G Cojoc, S Girardo… - Journal of …, 2018 - Wiley Online Library
Cells alter the path of light, a fact that leads to well‐known aberrations in single cell or tissue
imaging. Optical diffraction tomography (ODT) measures the biophysical property that …

[HTML][HTML] Full-field interferometric imaging of propagating action potentials

T Ling, KC Boyle, G Goetz, P Zhou, Y Quan… - Light: Science & …, 2018 - nature.com
Currently, cellular action potentials are detected using either electrical recordings or
exogenous fluorescent probes that sense the calcium concentration or transmembrane …

[HTML][HTML] Immature and mature bone marrow-derived dendritic cells exhibit distinct intracellular mechanical properties

A Leblanc-Hotte, C Audiger, G Chabot-Roy… - Scientific Reports, 2023 - nature.com
Dendritic cells (DCs) patrol the organism at an immature stage to detect the presence of
pathogens. Once activated, these mature DCs reach the lymph nodes to activate antigen …

Reply to: Methodological concerns and lack of evidence for single-synapse RNA-seq

M Niu, C Zong - Nature Biotechnology, 2023 - nature.com
The commentary by BD Hobson and E. Herzog 1 has raised major criticism about our
approach to isolating synaptosomes. We believe that the two authors have misunderstood …