Fungi do not have different sexes characterized by specific morphological organs but control
sexual development by physiological differences defined by mating types. While other …

Mating in Saccharomyces cerevisiae is initiated by the secretion of diffusible peptide
pheromones that are recognized by G protein-coupled receptors (GPCR). This review …

We outline a powerful method for the directed evolution of integral membrane proteins in the
inner membrane of Escherichia coli. For a mammalian G protein-coupled receptor, we …

The fungal class D1 G-protein-coupled receptor (GPCR) Ste2 has a different arrangement of
transmembrane helices compared with mammalian GPCRs and a distinct mode of coupling …

G protein-coupled receptors (GPCRs) regulate diverse biological processes in eukaryotes
and such conservation allows an almost unrestricted interchange of signalling components …

All G protein-coupled receptors (GPCRs) share a common seven TM helix architecture and
the ability to activate heterotrimeric G proteins. Nevertheless, these receptors have widely …

According to receptor theory, the effect of a ligand depends on the amount of agonist–
receptor complex. Therefore, changes in receptor abundance should have quantitative …

Oligomerization of G protein-coupled receptors is commonly observed, but the functional
significance of oligomerization for this diverse family of receptors remains poorly …

The goal of this study was to functionally express the three Gq‐coupled muscarinic receptor
subtypes, M1, M3 and M5, in yeast (Saccharomyces cerevisiae). Transformation of yeast …

The yeast α-factor receptor encoded by the STE2 gene is a member of the extended family
of G protein coupled receptors (GPCRs) involved in a wide variety of signal transduction …