Based on engineered or bacterial nucleases, the development of genome editing
technologies has opened up the possibility of directly targeting and modifying genomic …

Reverse genetics in model organisms such as Drosophila melanogaster, Arabidopsis
thaliana, zebrafish and rats, efficient genome engineering in human embryonic stem and …

A long-term goal in the field of restriction-modification enzymes has been to generate
restriction endonucleases with novel sequence specificities by mutating or engineering …

The field of bioinorganic chemistry is at a propitious stage of development. Ever more
complex metallobiomolecules are isolated and purified, physical methodologies and …

Specific interactions between proteins and DNA are fundamental to many biological
processes. In this review, we provide a revised view of protein-DNA interactions that …

Zinc ions are key structural components of a large number of proteins. The binding of zinc
stabilizes the folded conformations of domains so that they may facilitate interactions …

An account is given of the discovery of the classical Cys2His2 zinc finger, arising from the
interpretation of biochemical studies on the interaction of the Xenopus protein transcription …

▪ Abstract Cys2His2 zinc fingers are one of the most common DNA-binding motifs found in
eukaryotic transcription factors. These proteins typically contain several fingers that make …

▪ Abstract Cys2His2 zinc finger proteins offer a stable and versatile framework for the design
of proteins that recognize desired target sites on double-stranded DNA. Individual fingers …

On the basis of a structural analysis of 240 protein-DNA complexes contained in the Protein
Data Bank (PDB), we have classified the DNA-binding proteins involved into eight different …