Synergistic advances in optical physics, probe design, molecular biology, labeling
techniques and computational analysis have propelled fluorescence imaging into new …

Single-molecule fluorescence spectroscopy and super-resolution microscopy are important
elements of the ongoing technical revolution to reveal biochemical and cellular processes in …

Photoinduced reactions play an important role in the photocycle of fluorescent proteins from
the green fluorescent protein (GFP) family. Among such processes are photoisomerization …

Radiation damage inflicted during diffraction data collection in macromolecular
crystallography has re-emerged in the last decade as a major experimental and …

Sample fixation by vitrification is critical for the optimal structural preservation of
biomolecules and subsequent high-resolution imaging by cryo-correlative light and electron …

Radiation damage limits the accuracy of macromolecular structures in X-ray crystallography.
Cryogenic (cryo-) cooling reduces the global radiation damage rate and, therefore, became …

We demonstrate the use of cryogenic super-resolution correlative light and electron
microscopy (csCLEM) to precisely determine the spatial relationship between proteins and …

Phototransformable fluorescent proteins (FPs) have received considerable attention in
recent years, because they enable many new exciting modalities in fluorescence …

Enhanced Green Fluorescent Protein (EGFP) is a variant of wild-type Green Fluorescent
Protein from the jellyfish Aequorea victoria, whose mutations S65T and F64L increase …

Optical fluorescence microscopy plays a pivotal role in the exploration of biological structure
and dynamics, especially on live specimens. Progress in the field relies, on the one hand, on …