Considerations and best practices in animal science 16S ribosomal RNA gene sequencing microbiome studies
Microbiome studies in animal science using 16S rRNA gene sequencing have become
increasingly common in recent years as sequencing costs continue to fall and bioinformatic …
increasingly common in recent years as sequencing costs continue to fall and bioinformatic …
Host-microbiome interaction in lung cancer
Commensal microbiota has emerged as an essential biomarker and regulator of both
tumorigenesis and response to cancer therapy. However, our current knowledge about …
tumorigenesis and response to cancer therapy. However, our current knowledge about …
[HTML][HTML] Human gut colonisation may be initiated in utero by distinct microbial communities in the placenta and amniotic fluid
Interaction with intestinal microbes in infancy has a profound impact on health and disease
in later life through programming of immune and metabolic pathways. We collected maternal …
in later life through programming of immune and metabolic pathways. We collected maternal …
Refinement of 16S rRNA gene analysis for low biomass biospecimens
R Villette, G Autaa, S Hind, JB Holm… - Scientific Reports, 2021 - nature.com
High-throughput phylogenetic 16S rRNA gene analysis has permitted to thoroughly delve
into microbial community complexity and to understand host-microbiota interactions in …
into microbial community complexity and to understand host-microbiota interactions in …
ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples
I Abellan-Schneyder, AJ Schusser, K Neuhaus - BMC microbiology, 2021 - Springer
Background One limiting factor of short amplicon 16S rRNA gene sequencing approaches is
the use of low DNA amounts in the amplicon generation step. Especially for low-biomass …
the use of low DNA amounts in the amplicon generation step. Especially for low-biomass …
[HTML][HTML] Culture-and non-culture-based approaches reveal unique features of the ocular microbiome in dry eye patients
M Naqvi, F Fineide, TP Utheim, C Charnock - The Ocular Surface, 2024 - Elsevier
Purpose The purpose of this study was to investigate the ocular microbiome in individuals
with dry eye disease and to identify features of their ocular microbiome of possible health …
with dry eye disease and to identify features of their ocular microbiome of possible health …
Succession and determinants of the early life nasopharyngeal microbiota in a South African birth cohort
S Claassen-Weitz, S Gardner-Lubbe, Y Xia… - Microbiome, 2023 - Springer
Background Bacteria colonizing the nasopharynx play a key role as gatekeepers of
respiratory health. Yet, dynamics of early life nasopharyngeal (NP) bacterial profiles remain …
respiratory health. Yet, dynamics of early life nasopharyngeal (NP) bacterial profiles remain …
Benchmarking laboratory processes to characterise low-biomass respiratory microbiota
R Hasrat, J Kool, WAA de Steenhuijsen Piters… - Scientific reports, 2021 - nature.com
The low biomass of respiratory samples makes it difficult to accurately characterise the
microbial community composition. PCR conditions and contaminating microbial DNA can …
microbial community composition. PCR conditions and contaminating microbial DNA can …
Microbial communities of the upper respiratory tract in mild and severe COVID-19 patients: A possible link with the disease course
JS Galeeva, EV Starikova, DE Fedorov… - Frontiers in …, 2023 - frontiersin.org
The microbiota of the respiratory tract remains a relatively poorly studied subject. At the
same time, it is involved in modulating the immune response to infectious agents in the host …
same time, it is involved in modulating the immune response to infectious agents in the host …
[HTML][HTML] Sample collection methods in upper gastrointestinal research
HJ Yang, SI Seo, J Lee, CW Huh… - Journal of Korean …, 2023 - synapse.koreamed.org
In recent years, significant translational research advances have been made in the upper
gastrointestinal (GI) research field. Endoscopic evaluation is a reasonable option for …
gastrointestinal (GI) research field. Endoscopic evaluation is a reasonable option for …