Selection and validation of optimal RT-qPCR reference genes for the normalization of gene expression under different experimental conditions in Lindera megaphylla

H Liu, J Liu, P Chen, X Zhang, K Wang, J Lu, Y Li - Plants, 2023 - mdpi.com
Lindera megaphylla, a broad-leaved evergreen that is used as a landscape ornamental
plant and medicinal plant, is an ecologically important and dominant tree species. However …

Selection and validation of reference genes for measuring gene expression in Piper species at different life stages using RT-qPCR analysis

LF de Oliveira, AR Piovezani, DA Ivanov… - Plant Physiology and …, 2022 - Elsevier
The secondary metabolism of Piper species is known to produce a myriad of natural
products from various biosynthetic pathways which, represent a rich source of previously …

Validation of reference gene stability for normalization of RT-qPCR in Phytophthora capsici Leonian during its interaction with Piper nigrum L.

S Vijayakumar, M Sakuntala - Scientific Reports, 2024 - nature.com
The selection of stable reference genes for the normalization of reverse transcription
quantitative real-time PCR (RT-qPCR) is generally overlooked despite being the crucial …

Reference gene selection for RT-qPCR normalization of strawberry at various organs, different growing stages of fruits, and salt-stress treatment

H Topcu - Turkish Journal of Agriculture and Forestry, 2022 - journals.tubitak.gov.tr
Abstract Strawberry (Fragaria x ananassa Duch.) is a favorite fruit of high economic value
due to its good taste and high nutrition ingredients. Strawberry is a fruit grown around the …

Analysis of reference genes stability and histidine kinase expression under cold stress in Cordyceps militaris

YN Liu, BY Liu, YC Ma, HL Yang, GQ Liu - Plos one, 2020 - journals.plos.org
The development of fungal fruiting bodies from a hyphal thallus is inducible under low
temperature (cold stress). The molecular mechanism has been subject to surprisingly few …

Identification and validation of reference genes for RT-qPCR analysis in Iris domestica under Cd stress

B Su, Z Li, H Liu, X Niu, X Zhao, Y Wu, Q Wang, Y Yuan… - Heliyon, 2024 - cell.com
Iris domestica is a widely used ornamental garden and important medicinal plant. Our
previous studies have shown that it exhibits significant uptake and translocation capacity …

Identification of resistance gene analogs involved in Phytophthora capsici recognition in black pepper (Piper nigrum L.)

EJ Suraby, D Prasath, KN Babu, M Anandaraj - Journal of Plant Pathology, 2020 - Springer
Black pepper (Piper nigrum L.) is an important spice crop with high economic value.
However, its production is severely hampered by the oomycete pathogen, Phytophthora …

Identification of suitable reference genes for quantitative reverse transcription PCR in Luffa (Luffa cylindrica)

G Zhao, M Wang, Y Gan, H Gong, J Li, X Zheng… - … and Molecular Biology …, 2022 - Springer
Reverse transcription real-time quantitative PCR is widely used to quantify gene expression.
Reference genes are usually used as internal controls to measure the target gene …

Evaluation of housekeeping gene expression stability in carnation (Dianthus caryophyllus)

W Yu, Y Tao, L Luo, J Hrovat, A Xue… - New Zealand Journal of …, 2021 - Taylor & Francis
The real-time quantitative reverse transcription PCR (RT-qPCR) is widely used for gene
expression analysis, owing to its advantages of high specificity, sensitivity and repeatability …