The nuclear factor kappa B (NFκB) family of transcription factors is a key regulator of immune
development, immune responses, inflammation, and cancer. The NFκB signaling system …

Two opposing models have been proposed to describe the function of the MYC oncoprotein
in shaping cellular transcriptomes: one posits that MYC amplifies transcription at all active …

Major features of transcription by human RNA polymerase II (Pol II) remain poorly defined
due to a lack of quantitative approaches for visualizing Pol II progress at nucleotide …

Protein-coding genes in eukaryotes are transcribed by RNA polymerase II (Pol II) and
introns are removed from pre-mRNA by the spliceosome. Understanding the time lag …

At each active protein-encoding gene, nascent RNA is tethered to the DNA axis by
elongating RNA polymerase II (Pol II) and is continuously altered by splicing and other …

An important step toward understanding gene regulation is the elucidation of the time
necessary for the completion of individual steps. Measurement of reaction rates can reveal …

RNA splicing is an essential part of eukaryotic gene expression. Although the mechanism of
splicing has been extensively studied in vitro, in vivo kinetics for the two-step splicing …

Alternative splicing represents an important level of the regulation of gene function in
eukaryotic organisms. It plays a critical role in virtually every biological process within an …

Alternative splicing is an important step in eukaryotic mRNA pre-processing which increases
the complexity of gene expression programs, but is frequently altered in disease. Previous …

Alternative splicing is a key step in eukaryotic gene expression that allows for the production
of multiple transcript and protein isoforms from the same gene. Even though splicing is …