Advances in targeted proteomics and applications to biomedical research

T Shi, E Song, S Nie, KD Rodland, T Liu, WJ Qian… - …, 2016 - Wiley Online Library
Targeted proteomics technique has emerged as a powerful protein quantification tool in
systems biology, biomedical research, and increasing for clinical applications. The most …

Toward an integrated pipeline for protein biomarker development

AP Drabovich, E Martínez-Morillo… - Biochimica et Biophysica …, 2015 - Elsevier
Protein biomarker development is a multidisciplinary task involving basic, translational and
clinical research. Integration of multidisciplinary efforts in a single pipeline is challenging …

Rational design and development of SARS-CoV-2 serological diagnostics by immunoprecipitation-targeted proteomics

Z Fu, Y Rais, D Dara, D Jackson… - Analytical …, 2022 - ACS Publications
Current design of serological tests utilizes conservative immunoassay approaches and is
focused on fast and convenient assay development, throughput, straightforward …

Redefining serological diagnostics with immunoaffinity proteomics

J Walter, Z Eludin, AP Drabovich - Clinical Proteomics, 2023 - Springer
Serological diagnostics is generally defined as the detection of specific human
immunoglobulins developed against viral, bacterial, or parasitic diseases. Serological tests …

Identification of psoriatic arthritis mediators in synovial fluid by quantitative mass spectrometry

D Cretu, I Prassas, P Saraon, I Batruch, R Gandhi… - Clinical proteomics, 2014 - Springer
Background Synovial fluid (SF) is a dynamic reservoir for proteins originating from the
synovial membrane, cartilage, and plasma, and may therefore reflect the pathophysiological …

Mapping isoform abundance and interactome of the endogenous TMPRSS2-ERG fusion protein by orthogonal immunoprecipitation–mass spectrometry assays

Z Fu, Y Rais, TA Bismar, ME Hyndman, XC Le… - Molecular & Cellular …, 2021 - ASBMB
TMPRSS2-ERG gene fusion, a molecular alteration found in nearly half of primary prostate
cancer cases, has been intensively characterized at the transcript level. However limited …

Identification of TEX101-associated Proteins Through Proteomic Measurement of Human Spermatozoa Homozygous for the Missense Variant rs35033974*[S]

C Schiza, D Korbakis, K Jarvi, EP Diamandis… - Molecular & Cellular …, 2019 - ASBMB
TEX101 is a germ-cell-specific protein and a validated biomarker of male infertility. Mouse
TEX101 was found essential for male fertility and was suggested to function as a cell surface …

Mass spectrometry-based proteomics in basic and translational research of SARS-CoV-2 coronavirus and its emerging mutants

Y Rais, Z Fu, AP Drabovich - Clinical Proteomics, 2021 - Springer
Molecular diagnostics of the coronavirus disease of 2019 (COVID-19) now mainly relies on
the measurements of viral RNA by RT-PCR, or detection of anti-viral antibodies by …

Multi-omics biomarker pipeline reveals elevated levels of protein-glutamine gamma-glutamyltransferase 4 in seminal plasma of prostate cancer patients*[S]

AP Drabovich, P Saraon, M Drabovich… - Molecular & Cellular …, 2019 - ASBMB
Seminal plasma, because of its proximity to prostate, is a promising fluid for biomarker
discovery and noninvasive diagnostics. In this study, we investigated if seminal plasma …

Quantitative tandem mass-spectrometry of skin tissue reveals putative psoriatic arthritis biomarkers

D Cretu, K Liang, P Saraon, I Batruch, EP Diamandis… - Clinical Proteomics, 2015 - Springer
Abstract Background Psoriatic arthritis (PsA) is a distinct inflammatory arthritis occurring in
30% of psoriasis patients. There is a high prevalence of undiagnosed PsA in psoriasis …