The eukaryotic cell is compartmentalized into subcellular niches, including membrane-
bound and membrane-less organelles. Proteins localize to these niches to fulfil their …

Integral membrane proteins (IMPs) lie at the critical junctions between intracellular
compartments and cells and their environment. As such, IMPs are in a unique position to …

Background Male infertility is a major problem for mammalian reproduction. However,
molecular details including the underlying mechanisms of male fertility are still not known. A …

Background Many agricultural species and their pathogens have sequenced genomes and
more are in progress. Agricultural species provide food, fiber, xenotransplant tissues …

Extraction of membrane proteins from biological membranes is usually accomplished with
the help of detergents. This unit describes the use of detergents to solubilize and purify …

Human embryonic stem cells (hESCs) are of immense interest in regenerative medicine as
they can self-renew indefinitely and can give rise to any adult cell type. Human embryonal …

Developments in subcellular fractionation strategies have provided the means to profile and
analyze the protein composition of organelles and cellular structures by proteomics. Here …

Defining the cell surface proteome has profound importance for understanding cell
differentiation and cell–cell interactions, as well as numerous pathogenic abnormalities …

Detergents have been widely used for the solubilization of membrane proteins and the
improvement of their digestion. In this paper, we have evaluated the application of sodium …

Since the sequencing of the genome and the development of high-throughput tools for the
exploration of functional elements of the genome, the chicken has reached model organism …