Thioesters, amides, and esters are common chemical building blocks in a wide array of
natural products. The formation of these bonds can be catalyzed in a variety of ways. For …

Aminoacyl-tRNA synthetases (aaRSs) are modular enzymes globally conserved in the three
kingdoms of life. All catalyze the same two-step reaction, ie, the attachment of a …

Leucyl-tRNA synthetase (LeuRS) produces error-free leucyl-tRNALeu by coordinating
translocation of the 3′ end of (mis-) charged tRNAs from its synthetic site to a separate …

Lipases have been widely used as biocatalysts for numerous biotechnological applications.
In an earlier paper, we presented experimental results showing significantly enhanced …

Tryptophanyl-tRNA Synthetase (TrpRS) Urzyme (fragments A and C), a 130-residue
construct containing only secondary structures positioning the HIGH and KMSKS active site …

A project‐based biophysical chemistry laboratory course, which is offered to the
biochemistry and molecular biology majors in their senior year, is described. In this course …

Dihydronicotinamide riboside quinone oxidoreductase 2 is known to catalyze a two-electron
reduction of quinone to hydroquinone using its cofactor, flavin adenine dinucleotide. Using …

DinB is one of two Y family polymerases in E. coli and is involved in copying damaged DNA.
DinB is specialized to bypass deoxyguanosine adducts that occur at the N2 position, with its …

Prolyl-tRNA synthetases (ProRSs) have been shown to activate both cognate and some
noncognate amino acids and attach them to specific tRNAPro substrates. For example …

Aminoacyl-tRNA synthetases are multidomain enzymes that catalyze covalent attachment of
amino acids to their cognate tRNA. Cross-talk between functional domains is a prerequisite …