Cullin-RING ubiquitin ligases (CRLs) are dynamic modular platforms that regulate myriad
biological processes through target-specific ubiquitylation. Our knowledge of this system …

Pathogen-associated molecular patterns (PAMPs) and danger-associated molecular
patterns (DAMPs) induce NLRP3 inflammasome activation, and subsequent formation of …

Ubiquitin (Ub) chain formation by homologous to E6AP C-terminus (HECT)-family E3 ligases
regulates vast biology, yet the structural mechanisms remain unknown. We used chemistry …

Activity‐based E2 conjugating enzyme (E2)‐ubiquitin (Ub) probes have recently emerged as
effective tools for studying the molecular mechanism of E3 ligase (E3)‐catalyzed …

E3 ubiquitin ligases, in collaboration with E2 ubiquitin-conjugating enzymes, modify proteins
with poly-ubiquitin chains. Cullin-RING ligase (CRL) E3s use Cdc34/UBE2R-family E2s to …

Chloroplasts are plant organelles responsible for photosynthesis and environmental
sensing. Most chloroplast proteins are imported from the cytosol through the translocon at …

Lys ubiquitination is catalysed by E3 ubiquitin ligases and is central to the regulation of
protein stability and cell signalling in normal and disease states. There are gaps in our …

Studying the structural aspects of proteins within sub-cellular compartments is of growing
interest. Dynamic nuclear polarization supported solid-state NMR (DNP-ssNMR) is uniquely …

Cullin-RING ligases (CRLs) ubiquitylate specific substrates selected from other cellular
proteins. Substrate discrimination and ubiquitin transferase activity were thought to be strictly …

Posttranslational modifications, typically small chemical tags attached on amino acids
following protein biosynthesis, have a profound effect on protein structure and function …