Electrospray ionization (ESI) is an essential technique for transferring proteins from solution
into the gas phase for mass spectrometry and ion mobility spectrometry. The mechanisms …

Mass spectrometry based proteomics is an indispensable tool for almost all research areas
relevant for the understanding of proteolytic processing, ranging from the identification of …

Membrane proteins play critical biochemical roles but remain challenging to study. Recently,
native or nondenaturing mass spectrometry (MS) has made great strides in characterizing …

The precise relationship between native gas-phase protein ion structure, charge,
desolvation, and activation remains elusive. Much evidence supports the Charge Residue …

Integral to advances in mass spectrometry (MS) are developments in ionization technology,
which is constantly evolving because of advances in fundamental understanding …

The use of theta-glass emitters and mass spectrometry to monitor reactions that occur as fast
as one μs is demonstrated. Acidified aqueous solutions containing unfolded proteins are …

Excess protons in water exhibit unique transport properties because they can rapidly hop
along H-bonded water wires. Considerable progress has been made in unraveling this …

Collision-induced unfolding (CIU) of protein ions and their noncovalent complexes offers
relatively rapid access to a rich portfolio of biophysical information, without the need to tag or …

The ability to transfer intact proteins and protein complexes into the gas phase by
electrospray ionization (ESI) has opened up numerous mass spectrometry (MS)-based …

Supercharging electrospray ionization can be a powerful tool for increasing charge states in
mass spectra and generating unfolded ion structures, yet key details of its mechanism …