CRISPR/Cas9-based genome editing for disease modeling and therapy: challenges and opportunities for nonviral delivery

HX Wang, M Li, CM Lee, S Chakraborty… - Chemical …, 2017 - ACS Publications
Genome editing offers promising solutions to genetic disorders by editing DNA sequences
or modulating gene expression. The clustered regularly interspaced short palindromic …

Energy transfer with semiconductor quantum dot bioconjugates: a versatile platform for biosensing, energy harvesting, and other developing applications

N Hildebrandt, CM Spillmann, WR Algar, T Pons… - Chemical …, 2017 - ACS Publications
Luminescent semiconductor quantum dots (QDs) are one of the more popular nanomaterials
currently utilized within biological applications. However, what is not widely appreciated is …

Microfluidic nanomaterials: From synthesis to biomedical applications

K Illath, S Kar, P Gupta, A Shinde, S Wankhar… - Biomaterials, 2022 - Elsevier
Microfluidic platforms gain popularity in biomedical research due to their attractive inherent
features, especially in nanomaterials synthesis. This review critically evaluates the current …

Evaluation of novel cationic gene based liposomes with cyclodextrin prepared by thin film hydration and microfluidic systems

H Elsana, TOB Olusanya, J Carr-Wilkinson, S Darby… - Scientific reports, 2019 - nature.com
In gene delivery, non-viral vectors have become the preferred carrier system for DNA
delivery. They can overcome major viral issues such as immunogenicity and mutagenicity …

Non-viral delivery of CRISPR–Cas9 complexes for targeted gene editing via a polymer delivery system

J O'Keeffe Ahern, I Lara-Sáez, D Zhou, R Murillas… - Gene therapy, 2022 - nature.com
Recent advances in molecular biology have led to the CRISPR revolution, but the lack of an
efficient and safe delivery system into cells and tissues continues to hinder clinical …

Droplet microfluidics for synthetic biology

PC Gach, K Iwai, PW Kim, NJ Hillson, AK Singh - Lab on a Chip, 2017 - pubs.rsc.org
Synthetic biology is an interdisciplinary field that aims to engineer biological systems for
useful purposes. Organism engineering often requires the optimization of individual genes …

In situ bone tissue engineering using gene delivery nanocomplexes

A Malek-Khatabi, HA Javar, E Dashtimoghadam… - Acta biomaterialia, 2020 - Elsevier
Gene delivery offers promising outcomes for functional recovery or regeneration of lost
tissues at cellular and tissue levels. However, more efficient carriers are needed to safely …

Biomaterials meet microfluidics: from synthesis technologies to biological applications

J Ma, Y Wang, J Liu - Micromachines, 2017 - mdpi.com
Microfluidics is characterized by laminar flow at micro-scale dimension, high surface to
volume ratio, and markedly improved heat/mass transfer. In addition, together with …

Exploring the role of polymer structure on intracellular nucleic acid delivery via polymeric nanoparticles

CJ Bishop, KL Kozielski, JJ Green - Journal of Controlled Release, 2015 - Elsevier
Intracellular nucleic acid delivery has the potential to treat many genetically-based diseases,
however, gene delivery safety and efficacy remains a challenging obstacle. One promising …

Bioreducible polycations in nucleic acid delivery: past, present, and future trends

D Oupický, J Li - Macromolecular bioscience, 2014 - Wiley Online Library
Polycations that are degradable by reduction of disulfide bonds are developed for
applications in delivery of nucleic acids. This Feature Article surveys methods of synthesis of …