Nanoarray digital polymerase chain reaction with high-resolution melt for enabling broad bacteria identification and pheno–molecular antimicrobial susceptibility test

P Athamanolap, K Hsieh, CM O'Keefe… - Analytical …, 2019 - ACS Publications
Toward combating infectious diseases caused by pathogenic bacteria, there remains an
unmet need for diagnostic tools that can broadly identify the causative bacteria and …

Integrated bacterial identification and antimicrobial susceptibility testing using PCR and high-resolution melt

P Athamanolap, K Hsieh, L Chen, S Yang… - Analytical …, 2017 - ACS Publications
Accurate and timely diagnostics are critical for managing bacterial infections. The current
gold standard, culture-based diagnostics, can provide clinicians with comprehensive …

Nested machine learning facilitates increased sequence content for large-scale automated high resolution melt genotyping

SI Fraley, P Athamanolap, BJ Masek, J Hardick… - Scientific reports, 2016 - nature.com
Abstract High Resolution Melt (HRM) is a versatile and rapid post-PCR DNA analysis
technique primarily used to differentiate sequence variants among only a few short …

Diagnostic performance of real time PCR and MALDI-TOF in the detection of nontuberculous mycobacteria from clinical isolates

E Kalaiarasan, K Thangavelu, K Krishnapriya… - Tuberculosis, 2020 - Elsevier
This study aimed to evaluate the performance of real-time PCR (qPCR) and MALDI-TOF for
accurate and timely detection of nontuberculous mycobacterium (NTM) from clinical isolates …

Development of a new High Resolution Melting (HRM) assay for identification and differentiation of Mycobacterium tuberculosis complex samples

P Landolt, R Stephan, S Scherrer - Scientific reports, 2019 - nature.com
The rapid identification and differentiation of members of the Mycobacterium tuberculosis
complex (MTBC) is essential to assess the potential zoonotic risk. Different available …

Development and assessment of multiplex high resolution melting assay as a tool for rapid single-tube identification of five Brucella species

KK Gopaul, J Sells, R Lee, SM Beckstrom-Sternberg… - BMC research …, 2014 - Springer
Background The zoonosis brucellosis causes economically significant reproductive
problems in livestock and potentially debilitating disease of humans. Although the causative …

Use of real time polymerase chain reaction for detection of M. tuberculosis, M. avium and M. kansasii from clinical specimens

A Bainomugisa, E Wampande, C Muchwa, J Akol… - BMC infectious …, 2015 - Springer
Background The incidence of M. tuberculosis (MTB) and non tuberculous Mycobacterium
species (NTMs) like M. avium and M. kansasii has increased due to Human …

Application of a new designed high resolution melting analysis for mycobacterial species identification

AD Khosravi, H Meghdadi, M Hashemzadeh, A Alami… - BMC microbiology, 2024 - Springer
The Non-tuberculous mycobacterial (NTM) isolates should be distinguished from
tuberculosis and identified at the species level for choosing an appropriate treatment plan. In …

Differential identification of mycobacterial species using high-resolution melting analysis

AD Khosravi, M Hashemzadeh… - Frontiers in …, 2017 - frontiersin.org
Infections caused by non-tuberculous mycobacteria (NTM) is increasing wordwide. Due to
the difference in treatment of NTM infections and tuberculosis, rapid species identification of …

[PDF][PDF] Nontuberculous mycobacterial infections in children in the Czech Republic in the period 2003–2018

K Dolezalova, M Maly, J Wallenfels… - Biomed Pap Med …, 2021 - biomed.papers.upol.cz
Aim. Comparision of the incidence of cervical lymphadenitis caused by nontuberculous
mycobacteria in two equal time periods before and after the ending of widespread …