Hepatocytes operate in highly structured repeating anatomical units termed liver lobules.
Blood flow along the lobule radial axis creates gradients of oxygen, nutrients and hormones …

Polyploidy (or whole-genome duplication) is the condition of having more than two basic
sets of chromosomes. Polyploidization is well tolerated in many species and can lead to …

Fluorescence microscopy is a key driver of discoveries in the life sciences, with observable
phenomena being limited by the optics of the microscope, the chemistry of the fluorophores …

Recent development of improved tools and methods to analyse tissues at the three-
dimensional level has expanded our capacity to investigate morphogenesis of foetal liver …

Cellular lipid metabolism is a complex network process comprising dozens of enzymes,
multiple organelles and more than a thousand lipid species. Tracing metabolic reactions in …

Hepatocytes, the liver's predominant cells, perform numerous essential biological functions.
However, crucial events and regulators during hepatocyte maturation require in-depth …

Highlights•Cells in tissues operate in structured microenvironments.•Studying division of
labor in tissues requires spatially-resolved transcriptomics.•This is enabled by highly …

Single-cell RNA-seq reveals the role of pathogenic cell populations in development and
progression of chronic diseases. In order to expand our knowledge on cellular …

Exploring the cell biology of hepatocytes in vitro could be a powerful strategy to dissect the
molecular mechanisms underlying the structure and function of the liver in vivo. However …

Objectives Polyploidy is a fascinating characteristic of liver parenchyma. Hepatocyte
polyploidy depends on the DNA content of each nucleus (nuclear ploidy) and the number of …