Early detection of viral pathogens by DNA-sensors in clinical samples, contaminated foods,
soil or water can dramatically improve clinical outcomes and reduce the socioeconomic …

Global population is predicted to approach 10 billion by 2050, an increase of over 2 billion
from today. To meet the demands of growing, geographically and socio-economically …

CRISPR-Cas12a is an RNA-guided, programmable genome editing enzyme found within
bacterial adaptive immune pathways. Unlike CRISPR-Cas9, Cas12a uses only a single …

Point-of-care testing for nucleic acid that combines amplification and readout is in great
demand. This report integrates recombinase-aided amplification (RAA) with the clustered …

Bacterial contamination accounts for more than half of food poisoning cases. Conventional
methods such as colony-counting and general polymerase chain reaction are time …

Cas12a2 is a CRISPR-associated nuclease that performs RNA-guided, sequence-
nonspecific degradation of single-stranded RNA, single-stranded DNA and double-stranded …

Control of CRISPR/Cas12a trans-cleavage is crucial for biosensor development. Here, we
show that small circular DNA nanostructures which partially match guide RNA sequences …

Developing assays that combine CRISPR/Cas and isothermal nucleic acid amplification has
become a burgeoning research area due to the novelty and simplicity of CRISPR/Cas and …

Mycoplasma pneumoniae (MP) is a one of most common pathogen in causing respiratory
infection in children and adolescents. Rapid and efficient diagnostic methods are crucial for …

The CRISPR/Cas12a system exhibits extraordinary capability in the field of biosensing and
molecular diagnosis due to its trans-cleavage ability. However, it is still desirable for precise …