The powerful CRISPR genome editing system is hindered by its off-target effects, and
existing computational tools achieved limited performance in genome-wide off-target …

The detection of biomolecules is the key to basic molecular research, diagnostics, drug
screening, and other biomedical applications. However, the existing detection techniques …

Phosphorothioates (PS) have proven their effectiveness in the area of therapeutic
oligonucleotides with applications spanning from cancer treatment to neurodegenerative …

In addition to the well-characterized B-form of DNA, duplex DNA can adopt various
conformations, such as A or Z-DNA. Though less common, these structures can be induced …

Abstract The stability of Watson–Crick paired RNA/DNA hybrids is important for designing
optimal oligonucleotides for ASO (Antisense Oligonucleotide) and CRISPR (Clustered …

CRISPR-Cas9 is the forefront technology for editing the genome. In this system, the Cas9
protein is programmed with guide RNAs to process DNA sequences that match the guide …

Sequence-dependent properties of the DNA duplex have been accurately described using
extensive molecular dynamics simulations. The RNA duplex meanwhile─ which is typically …

Changes in the structure of double-stranded (ds) DNA with temperature affect processes in
thermophilic organisms and are important for nanotechnological applications. Here we …

The reprogrammable CRISPR/Cas9 genome editing tool's growing popularity is hindered by
unwanted off-target effects. Efforts have been directed toward designing efficient guide …

We propose an approach to help interpret polymer force–extension curves that exhibit
plateau regimes. When coupled to a bead–spring dynamic model, the approach accurately …