There has been rapid growth in the use of Drosophila and other invertebrate systems to
dissect mechanisms governing metabolism. New assays and approaches to physiology …

Whole-body health relies on complex inter-organ signalling networks that enable organisms
to adapt to environmental perturbations and to changes in tissue homeostasis. The intestine …

The ability to independently control gene expression in two different tissues in the same
animal is emerging as a major need, especially in the context of inter-organ communication …

Conditional gene regulation in Drosophila through binary expression systems like the LexA-
LexAop system provides a superb tool for investigating gene and tissue function. To …

Conditional expression of short hairpin RNAs with binary genetic systems is an
indispensable tool for studying gene function. Addressing mechanisms underlying cell–cell …

In vivo genome editing with clustered regularly interspaced short palindromic repeats
(CRISPR)/Cas9 generates powerful tools to study gene regulation and function. We revised …

The ability to drive expression of exogenous genes in different tissues and cell types, under
the control of specific enhancers, has been crucial for discovery in biology. While many …

CRISPR/Cas9 methods are a powerful in vivo approach to edit the genome of Drosophila
melanogaster. To convert existing Drosophila GAL4 lines to LexA driver lines in a secondary …

Conditional gene regulation in Drosophila through binary expression systems like the LexA-
LexAop system provides a superb tool for investigating gene and tissue function. To …

Genome editing in vivo with CRISPR/Cas9 generates powerful tools to study gene
regulation and function. We developed CRISPR-based methods that permitted secondary …