Plant molecular farming of virus‐like nanoparticles as vaccines and reagents

EP Rybicki - Wiley Interdisciplinary Reviews: Nanomedicine …, 2020 - Wiley Online Library
The use of plants for the production of virus‐like nanoparticles (VNPs) dates back to
separating natural empty capsids of plant viruses from whole virions nearly 70 years ago …

Recombinant protein-based viral disease diagnostics in veterinary medicine

V Balamurugan, G Venkatesan, A Sen… - Expert Review of …, 2010 - Taylor & Francis
Identification of pathogens or antibody response to pathogens in human and animals
modulates the treatment strategies for naive population and subsequent infections …

History and promise of plant-made vaccines for animals

E Rybicki - Prospects of plant-based vaccines in veterinary …, 2018 - Springer
Plant-made vaccines are now a well-established and well-tested concept in veterinary
medicine—yet the only product so far licenced was never produced commercially. This is …

Assessment of recombinant beak and feather disease virus capsid protein as a vaccine for psittacine beak and feather disease

N Bonne, P Shearer, M Sharp… - Journal of General …, 2009 - microbiologyresearch.org
Beak and feather disease virus (BFDV) is a significant pathogen of wild Australasian and
African psittacine birds. We assessed the immunogenicity of recombinant BFDV capsid …

Beak and feather disease virus infection in cockatiels (Nymphicus hollandicus)

PL Shearer, N Bonne, P Clark, M Sharp… - Avian pathology, 2008 - Taylor & Francis
Psittacine beak and feather disease is known to occur in a wide range of psittacine species;
however, there are no scientific or credible anecdotal reports of psittacine beak and feather …

A quantitative, real-time polymerase chain reaction assay for beak and feather disease virus

PL Shearer, M Sharp, N Bonne, P Clark… - Journal of Virological …, 2009 - Elsevier
PCR-based assays for the detection of BFDV DNA are in widespread use throughout the
world. Quantitative real-time PCR assays are extremely sensitive and have the advantages …

A blocking ELISA for the detection of antibodies to psittacine beak and feather disease virus (BFDV)

PL Shearer, M Sharp, N Bonne, P Clark… - Journal of virological …, 2009 - Elsevier
Currently, the only diagnostic test available routinely for the sero-diagnosis of BFDV is the
haemagglutination-inhibition (HI) assay. This test, whilst useful and applicable to samples …

Recombinant expression of beak and feather disease virus capsid protein and assembly of virus-like particles in Nicotiana benthamiana

GL Regnard, EP Rybicki, II Hitzeroth - Virology journal, 2017 - Springer
Background Beak and feather disease virus (BFDV) is an important disease causing agent
affecting psittacines. BFDV is highly infectious and can present as acute, chronic or …

An efficient approach for recombinant expression and purification of the viral capsid protein from beak and feather disease virus (BFDV) in Escherichia coli

S Sarker, SA Ghorashi, CMD Swarbrick… - Journal of virological …, 2015 - Elsevier
Structural insights into the biology of viruses such as beak and feather disease virus (BFDV)
which do not replicate in cell cultures are increasingly reliant on recombinant methods for …

Differential expression of two isolates of beak and feather disease virus capsid protein in Escherichia coli

EI Patterson, CMD Swarbrick, N Roman… - Journal of virological …, 2013 - Elsevier
Expression of recombinant beak and feather disease virus (BFDV) capsid-associated
protein (Cap) has relied on inefficient techniques that typically produce low yields or use …