The immune system varies in cell types, states, and locations. The complex networks,
interactions, and responses of immune cells produce diverse cellular ecosystems composed …

Blood and immune cells derive from multipotent hematopoietic stem cells (HSCs).
Classically, stem and progenitor populations have been considered discrete homogeneous …

Single-cell RNA sequencing (scRNA-seq) is a powerful approach for reconstructing cellular
differentiation trajectories. However, inferring both the state and direction of differentiation is …

Regulation of hematopoiesis during human development remains poorly defined. Here we
applied single-cell RNA sequencing (scRNA-seq) and single-cell assay for transposase …

Single-cell RNA sequencing (scRNA-seq) offers new possibilities to address biological and
medical questions. However, systematic comparisons of the performance of diverse scRNA …

Embryonic development is a crucial period in the life of a multicellular organism, during
which limited sets of embryonic progenitors produce all cells in the adult body. Determining …

Technological advances have made it possible to measure spatially resolved gene
expression at high throughput. However, methods to analyze these data are not established …

Single-cell genomics technology has transformed our understanding of complex cellular
systems. However, excessive cost and a lack of strategies for the purification of newly …

Vascular smooth muscle cells (VSMCs) show pronounced heterogeneity across and within
vascular beds, with direct implications for their function in injury response and …

Ever since hematopoietic stem cells (HSCs) were first identified half a century ago, their
differentiation roadmap has been extensively studied. The classical model of hematopoiesis …