The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome
editing, coupled with advances in computing and imaging capabilities, has initiated a new …

The human microbiome is composed of a collection of dynamic microbial communities that
inhabit various anatomical locations in the body. Accordingly, the coevolution of the …

Cell-based therapeutics are an emerging modality with the potential to treat many currently
intractable diseases through uniquely powerful modes of action. Despite notable recent …

A technique for chromosomal insertion of large DNA segments is much needed in plant
breeding and synthetic biology to facilitate the introduction of desired agronomic traits and …

Programmable genome integration of large, diverse DNA cargo without DNA repair of
exposed DNA double-strand breaks remains an unsolved challenge in genome editing. We …

CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …

The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …

Feeding the ever-growing population is a major challenge, especially in light of rapidly
changing climate conditions. Genome editing is set to revolutionize plant breeding and …

IscB proteins are putative nucleases encoded in a distinct family of IS200/IS605 transposons
and are likely ancestors of the RNA-guided endonuclease Cas9, but the functions of IscB …

CRISPR–Cas systems provide resistance against foreign mobile genetic elements and have
a wide range of genome editing and biotechnological applications. In this Review, we …