Biochemical basis of DNA replication fidelity
MF Goodman, S Creighton, LB Bloom… - Critical reviews in …, 1993 - Taylor & Francis
DNA polymerase is the critical enzyme maintaining genetic integrity during DNA replication.
Individual steps in the replication process that contribute to DNA synthesis fidelity include …
Individual steps in the replication process that contribute to DNA synthesis fidelity include …
DNA polymerase proofreading: Multiple roles maintain genome stability
LJ Reha-Krantz - Biochimica et Biophysica Acta (BBA)-Proteins and …, 2010 - Elsevier
DNA polymerase proofreading is a spell-checking activity that enables DNA polymerases to
remove newly made nucleotide incorporation errors from the primer terminus before further …
remove newly made nucleotide incorporation errors from the primer terminus before further …
PCR Fidelity of Pfu DNA Polymerase and Other Thermostable DNA Polymerases
J Cline, JC Braman, HH Hogrefe - Nucleic acids research, 1996 - academic.oup.com
The replication fidelities of Pfu, Taq, Vent, Deep Vent and UlTma DNA polymerases were
compared using a PCR-based forward mutation assay. Average error rates (mutation …
compared using a PCR-based forward mutation assay. Average error rates (mutation …
Lack of evidence for proofreading mechanisms associated with an RNA virus polymerase
DA Steinhauer, E Domingo, JJ Holland - Gene, 1992 - Elsevier
The in vitro fidelity of the virion-associated RNA polymerase of vesicular stomatitis virus was
quantitated for a single conserved viral RNA site and the usual high in vitro base …
quantitated for a single conserved viral RNA site and the usual high in vitro base …
[HTML][HTML] Fidelity of phi 29 DNA polymerase. Comparison between protein-primed initiation and DNA polymerization.
JA Esteban, M Salas, L Blanco - Journal of Biological Chemistry, 1993 - Elsevier
Phi 29 DNA polymerase is able to catalyze two different synthetic reactions: protein-primed
initiation and DNA polymerization. We have studied the fidelity of phi 29 DNA polymerase …
initiation and DNA polymerization. We have studied the fidelity of phi 29 DNA polymerase …
[HTML][HTML] DNA polymerase insertion fidelity. Gel assay for site-specific kinetics.
MS Boosalis, J Petruska, MF Goodman - Journal of Biological Chemistry, 1987 - Elsevier
A quantitative assay based on gel electrophoresis is described to measure nucleotide
insertion kinetics at an arbitrary DNA template site. The assay is used to investigate kinetic …
insertion kinetics at an arbitrary DNA template site. The assay is used to investigate kinetic …
Kinetic mechanism whereby DNA polymerase I (Klenow) replicates DNA with high fidelity
RD Kuchta, P Benkovic, SJ Benkovic - Biochemistry, 1988 - ACS Publications
Department of Chemistry, 152 Davey Laboratory, The Pennsylvania State University,
University Park, Pennsylvania 16802 Received December 30, 1987; Revised Manuscript …
University Park, Pennsylvania 16802 Received December 30, 1987; Revised Manuscript …
Hydrogen bonding revisited: geometric selection as a principal determinant of DNA replication fidelity
MF Goodman - Proceedings of the National Academy of …, 1997 - National Acad Sciences
That hydrogen bonds play a central role in forming Watson–Crick (W–C) AT and GC base
pairs is a fundamental paradigm dating from the discovery of the structure of DNA in 1953. In …
pairs is a fundamental paradigm dating from the discovery of the structure of DNA in 1953. In …
[HTML][HTML] Base mispair extension kinetics. Comparison of DNA polymerase alpha and reverse transcriptase.
LV Mendelman, J Petruska, MF Goodman - Journal of Biological Chemistry, 1990 - Elsevier
A polyacrylamide gel assay is used to measure the kinetics of adding a single
deoxyribonucleotide onto either a correctly matched or mismatched primer 3'terminus (on …
deoxyribonucleotide onto either a correctly matched or mismatched primer 3'terminus (on …
[19] Gel fidelity assay measuring nucleotide misinsertion, exonucleolytic proofreading, and lesion bypass efficiencies
S Creighton, LB Bloom, MF Goodman - Methods in enzymology, 1995 - Elsevier
Publisher Summary The fidelity of DNA synthesis depends on polymerase-primer template-
nucleotide interactions that can alter fidelity at different locations along a primer-template …
nucleotide interactions that can alter fidelity at different locations along a primer-template …