Abstract Uncultivated Bacteria and Archaea account for the vast majority of species on Earth,
but obtaining their genomes directly from the environment, using shotgun sequencing, has …

Advances in sequencing technologies and bioinformatics tools have dramatically increased
the recovery rate of microbial genomes from metagenomic data. Assessing the quality of …

Our view of genome size in Archaea and Bacteria has remained skewed as the data has
been dominated by genomes of microorganisms that have been cultivated under laboratory …

The role of the Arctic Ocean ecosystem in climate regulation may depend on the responses
of marine microorganisms to environmental change. We applied genome-resolved …

The recovery of metagenome-assembled genomes (MAGs) from metagenomic data has
recently become a common task for microbial studies. The strengths and limitations of the …

The phylum Actinobacteria includes important human pathogens like Mycobacterium
tuberculosis and Corynebacterium diphtheriae and renowned producers of secondary …

Eukaryotic genomes are known to have garnered innovations from both archaeal and
bacterial domains but the sequence of events that led to the complex gene repertoire of …

Amplicon sequencing of the 16S rRNA gene is the predominant method to quantify microbial
compositions and to discover novel lineages. However, traditional short amplicons often do …

With advances in DNA sequencing and miniaturized molecular biology workflows, rapid and
affordable sequencing of single-cell genomes has become a reality. Compared to 16S rRNA …

Diarrheagenic Escherichia coli, collectively known as DEC, is a leading cause of diarrhea,
particularly in children in low-and middle-income countries. Diagnosing infections caused by …