The development of new biocatalytic systems to replace the chemical catalysts, with suitable
characteristics in terms of efficiency, stability under high temperature reactions and in the …

The combination of computational and directed evolution methods has proven a winning
strategy for protein engineering. We refer to this approach as computer-aided protein …

The haloarchaeon Halorubrum lacusprofundi is among the few polyextremophilic organisms
capable of surviving in one of the most extreme aquatic environments on Earth, the Deep …

Abstract Bacillus gibsonii Alkaline Protease (BgAP) is a recently reported subtilisin protease
exhibiting activity and stability properties suitable for applications in laundry and dish …

Improving the thermostability of industrial enzymes is an important protein engineering
challenge. Point mutations, induced to increase thermostability, affect the structure and …

The computational study of large biomolecular complexes (molecular machines, cytoskeletal
filaments, etc.) is a formidable challenge facing computational biophysics and biology. To …

The psychrophilic and mesophilic endonucleases A (EndA) from Aliivibrio salmonicida
(VsEndA) and Vibrio cholera (VcEndA) have been studied experimentally in terms of the …

Alginate lyase has received extensive attention as an important tool for oligosaccharide
preparation, pharmaceutical production, and energy biotransformation. Noncatalytic module …

In proteins, a posttranslational deamidation process converts asparagine (Asn) and
glutamine (Gln) residues into negatively charged aspartic (Asp) and glutamic acid (Glu) …

The physicochemical bases for enzyme cold-adaptation remain elusive. The current view is
that psychrophilic enzymes are often characterized by enhanced flexibility at low …