Temperature-resistant and solvent-tolerant lipases as industrial biocatalysts: Biotechnological approaches and applications
The development of new biocatalytic systems to replace the chemical catalysts, with suitable
characteristics in terms of efficiency, stability under high temperature reactions and in the …
characteristics in terms of efficiency, stability under high temperature reactions and in the …
[HTML][HTML] Computer-aided protein directed evolution: a review of web servers, databases and other computational tools for protein engineering
The combination of computational and directed evolution methods has proven a winning
strategy for protein engineering. We refer to this approach as computer-aided protein …
strategy for protein engineering. We refer to this approach as computer-aided protein …
[HTML][HTML] Understanding high-salt and cold adaptation of a polyextremophilic enzyme
The haloarchaeon Halorubrum lacusprofundi is among the few polyextremophilic organisms
capable of surviving in one of the most extreme aquatic environments on Earth, the Deep …
capable of surviving in one of the most extreme aquatic environments on Earth, the Deep …
Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution
R Martinez, F Jakob, R Tu, P Siegert… - Biotechnology and …, 2013 - Wiley Online Library
Abstract Bacillus gibsonii Alkaline Protease (BgAP) is a recently reported subtilisin protease
exhibiting activity and stability properties suitable for applications in laundry and dish …
exhibiting activity and stability properties suitable for applications in laundry and dish …
Understanding the Thermostability and Activity of Bacillus subtilis Lipase Mutants: Insights from Molecular Dynamics Simulations
Improving the thermostability of industrial enzymes is an important protein engineering
challenge. Point mutations, induced to increase thermostability, affect the structure and …
challenge. Point mutations, induced to increase thermostability, affect the structure and …
Optimal number of coarse-grained sites in different components of large biomolecular complexes
The computational study of large biomolecular complexes (molecular machines, cytoskeletal
filaments, etc.) is a formidable challenge facing computational biophysics and biology. To …
filaments, etc.) is a formidable challenge facing computational biophysics and biology. To …
[HTML][HTML] A comparative study of cold-and warm-adapted Endonucleases A using sequence analyses and molecular dynamics simulations
The psychrophilic and mesophilic endonucleases A (EndA) from Aliivibrio salmonicida
(VsEndA) and Vibrio cholera (VcEndA) have been studied experimentally in terms of the …
(VsEndA) and Vibrio cholera (VcEndA) have been studied experimentally in terms of the …
[HTML][HTML] Effects of Module Truncation of a New Alginate Lyase VxAly7C from Marine Vibrio xiamenensis QY104 on Biochemical Characteristics and Product …
L Tang, M Bao, Y Wang, Z Fu, F Han, W Yu - International Journal of …, 2022 - mdpi.com
Alginate lyase has received extensive attention as an important tool for oligosaccharide
preparation, pharmaceutical production, and energy biotransformation. Noncatalytic module …
preparation, pharmaceutical production, and energy biotransformation. Noncatalytic module …
Surface charge engineering of a Bacillus gibsonii subtilisin protease
F Jakob, R Martinez, J Mandawe, H Hellmuth… - Applied microbiology …, 2013 - Springer
In proteins, a posttranslational deamidation process converts asparagine (Asn) and
glutamine (Gln) residues into negatively charged aspartic (Asp) and glutamic acid (Glu) …
glutamine (Gln) residues into negatively charged aspartic (Asp) and glutamic acid (Glu) …
[HTML][HTML] Molecular motions and free-energy landscape of serine proteinase K in relation to its cold-adaptation: A comparative molecular dynamics simulation study …
P Sang, X Du, LQ Yang, ZH Meng, SQ Liu - RSC advances, 2017 - pubs.rsc.org
The physicochemical bases for enzyme cold-adaptation remain elusive. The current view is
that psychrophilic enzymes are often characterized by enhanced flexibility at low …
that psychrophilic enzymes are often characterized by enhanced flexibility at low …